摘要
本文采用新试剂2-(5-硝基-2-吡啶偶氮)-5-二乙氨基苯酚(5-NO_2-PADAP)为柱前衍生试剂,不需对离子试剂,用含40mmol/L 的 pH6.0的乙酸-乙酸钠缓冲溶液和10^(-4)mol/LEDTANa_2的甲醇-水溶液(67.5:32.5)为流动相,在 ODS 柱上,20min 内 HPLC 测定了 Fe(Ⅲ)、CO(Ⅱ)和 Ni(Ⅱ)的5-NO_2-PADAP 的络合物,这是同类衍生试剂中最简单流动相体系。当信噪比 SNR=2时,检出限分别为0.17、0.17和0.09ng/ml。是同类体系中灵敏度最高的。方法用于同时测定硝酸试剂和茶叶中的铁、钴和镍,结果良好。
The reversed-phase high-performance liquid chromatography of chelate of iron(Ⅱ),cobalt(Ⅱ)and nickel(Ⅱ)with new organic reagent 2-(5-nitro-2-pyridylazo)-5- diathylaminophenol on an ODS column was studied.Metanol-water(67.5∶32.5,V/V)contai nied 40mmol/L HAc-NaAc buffer solution(pH6.0)and 1×10^(-4)mol/L EDTANa_2 was used as mobile phase.Fe(Ⅲ),Co(Ⅱ)and Ni(Ⅱ)chelates were separated and determined within 20min.When signal noise ratio was 2,detection limit of Fe(Ⅲ),Co(Ⅱ)and Ni(Ⅱ) is 0.17,0.17 and 0.09ng/ml,respectively.This method has been applied to the determination of trace Fe,Co and Ni in nitric acid,hydrochloric acid and Chinese red tea samples.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1991年第11期1261-1264,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金
关键词
铁
钴
镍
测定
5-NO2-PADAP
RHLC
High-performance liquid chromatography
2-(5-Nitro-2-pyridylazo)-5-diethylaminophenol, Iron,Cobalt,Nickel, Spectrophotometry
