摘要
目的:研究绿原酸对大鼠肠道线粒体损伤的缓解作用。方法:采用H2O2诱导大鼠肠道线粒体氧化损伤,通过测定线粒体肿胀度、膜电位、丙二醛(MDA)含量、总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力,以及线粒体呼吸链复合物Ⅰ、Ⅳ、Ⅴ的活性,分析绿原酸对肠道线粒体损伤的缓解效果。结果:绿原酸可显著缓解H2O2造成的线粒体肿胀和膜电位的降低。绿原酸(0.032mg/mL和0.064mg/mL)显著降低MDA的含量,绿原酸(0.064mg/mL)能显著提高T-SOD、GSH-Px及复合物Ⅰ酶的活力。结论:绿原酸通过提高线粒体呼吸链复合物酶和抗氧化酶的活力,缓解线粒体H2O2的损伤。
This study was to explore the effect of chlorogenic acid on damaged rat intestinal mitochondria. Methods： Intestinal mitochondria of rats were treated with hydrogen peroxide. The mitochondrial swelling, the membrane potential, the concentration of MDA, T-SOD and GSH-Px activity, and the enzymatic activities of mitochondrial respiratory chain complexes I, IV, and V were compared. Results： CHA significantly inhibited mitochondrial swelling caused by hydrogen peroxide and reduced the membrane potential. CHA （0.032 mg/mL and 0.064 mg/mL） significantly decreased the concentration of MDA compared to damaged group. The enzymatic activity of T-SOD, GSH-Px and complex I in CHA group （0.064 mg/mL） were significantly increased compared to damaged group. Conclusion： CHA attenuated the mitochondrial damage induced by hydrogen peroxide through increasing mitochondrial respiratory chain complex enzymes and antioxidant enzymes activity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第21期349-353,共5页
Food Science
关键词
关键词
绿原酸
线粒体
氧化损伤
呼吸链复合物
Keywords： chlorogenic acid
mitochondrial
oxidative damage
respiratory chain complex
