摘要
目的 构建及鉴定重组腺病毒载体pAdxsi-GFP-Galectin-1.方法 通过Kpn I/BamH I双酶切从原始质粒获取半乳糖凝集素-1完整基因片段并连接到pSbuttle-CMV-EGFP重组穿梭载体；通过I-Ceu I＋I-Sce I双酶切获取CMV-GFP-Galectin-1完整基因片段并连接到pAdxsi载体上,得到重组腺病毒载体pAdxsi-GFP-Galectin-1.结果 通过基因测序、酶切鉴定证实目的基因克隆至重组腺病毒载体,目的基因序列与Genebank录入序列一致.结论 成功构建重组腺病毒载体pAdxsi-GFP-Galectin-1.
Objective To construct and identify the recombinant adenovirus vector pAdxsi-GFP-Galectin-1.Methods Whole sequence of galectin-1 was obtained from original plasmid double-digested with Kpn I/BamH I enzymes.Then Galectin-1 DNA segments were linked into pShuttle-CMV-EGFP vector to obtain recombinant pShuttle-GFP-Galectin-1 plasmid.Next,recombinant DNA sequences CMV-GFP-Galectin1were double-digested with I-Ceu I/I-Sce I enzymes and constructed into pShuttle-GFP-Galectin-1 vector to establish recombinant adenovirus vector pAdxsi-GFP-Galectin-1.Results Through endonuclease and gene sequencing the target gene was verified to be corrrctly cloned in adenvrius vector.Conclusion The recombinant adenovirus vector pAdxsi-GFP-Galectin-1 was successfully constructed.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第11期2315-2317,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81070387）
