摘要
目的：建立玳玳黄酮提取物的质量标准。方法：采用薄层色谱法鉴别玳玳黄酮提取物中的柚皮苷和新橙皮苷；采用紫外分光光度法测定玳玳黄酮提取物中总黄酮含量；采用高效液相色谱法同时检测玳玳黄酮提取物中柚皮苷和新橙皮苷的含量。结果：薄层色谱斑点清晰，易于识别，专属性强；紫外分光光度法新橙皮苷在4．44～26．64μg·mL-1范围内呈良好线性关系，平均加样回收率为99．92％（RSD=1．65％）；HPLC法柚皮苷在1.988—13．916μg·mL-1范围内呈良好线性关系，平均加样回收率为99．73％（RSD=1．56％），新橙皮苷在1．992～13．944μg·mL-1范围内呈良好线性关系，平均加样回收率为99．58％（RsD=1．89％）。结论：所建方法操作简便，结果准确，可有效控制玳玳黄酮提取物的质量。
Objective： To determine the quality standard of Dai Dai flavone extracts. Methods： TLC was applied to the identification of naringin and neohesperidin; UV-spectrophotometry was used to measure the content of total flavones, and HPLC to determine naring-in and neohesperidin simultaneously in Dai Dai flavone extracts. Results： The qualitative identification with TLC was specific; under the UV-speetrophotometry, neohcsperidin had a good linearity in the range of 4.44-26. 64μg·mL-1 , the average recovery was 99.92% （RSD = 1.65% ） ; under the HPLC,naringin had a good linearity in the range of 1. 988-13. 916 μg·mL-1 , the average recovery was 99. 73% （RSD = 1.56% ） , neohesperidin in the range of 1. 992-13. 944 μg·mL-1 , the average recovery was 99. 58% （ RSD = 1.89% , ）. Conclusions ： The methods were simple, accurate and reliable, can be well used in the quality control of Dai Dai flavone extracts.
出处
《中国药品标准》
CAS
2013年第5期337-341,共5页
Drug Standards of China
基金
福建省自然科学基金项目(2012J01386
2010J01189)
福建省发展和改革委员会产业技术开发项目(闽发改高技【2011】1598号)
