摘要
研究了聚乙二醇(PEG)为内标物的电喷雾(ESI)/飞行时间质谱(TOF-MS)准确质量测定方法,并应用于5个红霉素类抗生素质子化分子离子(MH^+)的质量测定.与理论值相比,相对误差均在5×10^(-6)以内.PEG可与K^+,Na^+,H^+等形成三种加合离子形式,通过选择适当的实验条件,控制PEG仅以其中的一种加合离子形式出现,这一特点扩大了其应用范围,使之可作为一种普适性的内标物.另外讨论了扫描质量范围、扫描速度等因素对测定结果的影响,并且比较了采用多峰校正法和两峰校正法的结果.结果表明,以PEG为内标的ESI/TOF质谱法可对不稳定碱性化合物的质子化分子离子(MH^+)进行准确质量测定,而且简便、快速.
Using polyethylene glycol(PEG) as internal reference, electrospray ionization(ESI)/time - of -flight mass spectrometry(TOF - MS) was developed to determine the accurate mass of the protonated molecular ions of five erythromycin analogue antibiotics. Compared with the theoretical values, all relative errors were less than 5×10-6. PEG can form three adduct ions with K+ , Na+ , H+ , respectively, and may appear with single kind of adduct ions in suitable experiment conditions. PEG was recommended as a general internal reference compound. Besides, two - peak calibration is preferred to the multi - peak calibration. Scan mass width and scan speed were also studied, 104 - 144amu 5 seconds was recommended. Using polyethylene glycol(PEG) as internal reference, ESI/TOF mass spectrometry can determine the accurate mass of the protonated molecular ions of the unsteady basic compounds. This method is simple and expeditious.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2000年第11期1452-1456,共5页
Acta Chimica Sinica
关键词
电喷雾
飞行时间质谱
红霉素类抗生素
质量测定
PEG internal reference method, electrospray ionization(ESI) , time -of- flight mass spectrometry (TOF- MS), erythromycin analogue antibiotic, accurate mass
