摘要
建立了测定环境水中痕量砷的荧光新方法 ,在 p H 6.5～ 7.5的缓冲介质中 ,利用 2′,7′-二氯荧光素 (DCF)作为荧光试剂 ,激发波长 λex=51 0 nm,发射波长 λem=52 8nm下 ,测定痕量砷 ,亚砷酸根浓度在 0 .2 5～ 3.50 μg/2 5m L范围内 ,相对荧光强度差值与亚砷酸根浓度呈线性关系 ,检测限为 0 .1 6μg/2 5m L,用于检测自来水和池塘水中的痕量砷 ,回收率为 95%～ 1 0 5%。
A fluorimetric method was developed for determination of arsenite with 2′,7′ dichlorofluorescein as fluorescent reagent ( λ ex =510 nm, λ em =528 nm) at pH 6.5～7.5. A linear calibration curve was obtained in the range 0.25～3.5 μg per 25 mL for arsenite with detection limit of 0.16 μg per 25 mL. The method was applied successfully to determination of arsenite in tap and pool water samples.
出处
《分析试验室》
CAS
CSCD
2000年第6期71-73,共3页
Chinese Journal of Analysis Laboratory
关键词
荧光法
砷
环境水
DCF
痕量分析
Fluorimetry
2′,7′ dichlorofluorescein
Arsenite
Environmental water sample
