摘要
应用基质辅助激光解吸电离飞行时间质谱（ＭＡＬＤＩ－ＴＯＦ－ＭＳ）法对长白山白眉蝮蛇蛇毒和纯化得到的两种蛇毒酶进行了研究，得到了它们的分子质量并验证了纯度。同时还考察了不同产地的蛇毒、蛇毒蛋白浓度以及基质对分析结果的影响。实验结果表明ＭＡＬＤ－ＴＯＦ－ＭＳ法是检测蛋白纯化过程和分析蛋白相对分子质量十分有效的手段。
The venom and two purified venom enzymes of Agkistrodon blomhoffii Ussuresis have been studied by MALDI - TOF - MS. The relative molecular masses of venom enzymes were determined and their purity tested. Effects of the haunt of snakeS, venom protein concentrations and substrate concen- trations were studied. The results indicate that MALSI - TOF - MS is one of the most effective tools for the assessment of protein purification processes and for the determination of protein molecular masses.
出处
《分析测试学报》
CAS
CSCD
2000年第6期81-83,共3页
Journal of Instrumental Analysis
