摘要
目的 研究Kruppel样因子6（KLF6）基因表达与胃癌发生、发展的关系.方法 采用免疫组织化学（SP法）与反转录-聚合酶链反应（RT-PCR）对73例胃癌及癌旁组织的KLF6蛋白与mRNA表达进行检测,分析KLF6表达与临床病理因素间的关系.结果 KLF6蛋白在胃癌及癌旁组织中的表达率分别为39.7％（29/73）、90.4％（66/73） （P＜0.01）,在低、高-中分化癌组织中的表达率分别为25.0％（10/40）、57.6％（19/33）（P＜0.01）.KLF6mRNA在胃癌及癌旁组织中表达水平（与GAPDH吸光度比值）分别为0.1586±0.1071、0.8899±0.0638 （P＜0.01）.结论 KLF6蛋白与mRNA在胃癌组织中呈低表达,且在低分化胃癌中低于高-中分化胃癌,表明KLF6与胃癌的发生、发展及分化关系密切,可作为判断胃癌生物学行为的重要指标.
Objective To investigate the correlation between Kruppel-like transcription factor 6 （KLF6） and gastric cancer.Methods By using immunohistochemistry （IHC） and reverse transcription PCR,the expression of KLF6 protein and mRNA in human gastric carcinoma specimens and adjacent tissues from 73 patients were examined.Results The KLF6 protein expression rates were 39.7 % （29/73） and 90.41%（66/73） in 73 cases of gastric cancer and adjacent tissues,The KLF6 protein expression was significantly lower in human gastric carcinomas than the adjacent tissues by chi-square test （P 〈 0.01）.The KLF6 expression rates were 25.0 % （10/40）,57.6 % （19/33） in poorly and well differentiated cancer.The difference between the two groups was significant （P 〈 0.01）.The optical density ratio of KLF6 mRNA were 0.1586±0.1071 and 0.8899±0.0638 in the gastric cancer and cancer adjacent tissue respectively,the difference between the two groups was significant （P 〈 0.01）.Conclusion KLF6 expression is low in gastric carcinoma,and moreover,KLF6 expression is significantly lower in poorly differentiated cancers than the well differentiated gastric cancers,there are negative correlation between KLF6 and gastric cancer,therefore KLF6 could be a useful marker for gastric cancer.
出处
《肿瘤研究与临床》
CAS
2013年第10期681-684,共4页
Cancer Research and Clinic
基金
合肥市医学重点学科建设计划
关键词
胃肿瘤
反转录聚合酶链反应
免疫组织化学
Kruppel样转录因子类
Stomach neoplasms
Reverse transcriptase polymerase chain reaction
Immunohistochemistry
Kruppel-like transcription factors
