摘要
目的:建立由脱氢表雄酮诱导的PCOS大鼠模型,观察健脾化痰方对PCOS大鼠胰岛素抵抗及慢性炎症因子水平的影响。方法:23日龄雌性SD大鼠皮下注射脱氢表雄酮6 mg/100 g体重/天,建立PCOS大鼠模型,成模大鼠随机分为4组:模型对照组、二甲双胍组、中药组(健脾化痰方)和二甲双胍+中药组,每组10只,分别给予相应药物进行干预,为期28 d。另设10只同龄SD大鼠为正常对照组。实验结束时,行腹腔糖耐量实验,测定0分、120分血糖及胰岛素水平;隔日将大鼠麻醉后下腔静脉取血,酶联免疫吸附法(ELISA)测定血清睾酮(T)、卵泡刺激素(FSH)、黄体生成素(LH)、性激素结合球蛋白(SHBG)、白细胞介素-6(IL-6)、内脂素(visfatin)和色素上皮衍生因子(PEDF)的水平,HE染色观察大鼠卵巢的组织学改变及排卵情况。结果与正常组比较,模型组大鼠血清T、LH、LH/FSH、血糖、胰岛素、HOMA-IR、IL-6、PEDF水平均显著升高(P<0.05),卵巢组织HE染色光镜下可见各级发育期卵泡及黄体减少,囊性扩张的卵泡增多,卵巢颗粒细胞层数减少;与模型组比较,各治疗组大鼠血清T、LH、LH/FSH、血糖、胰岛素、HOMA-IR、IL-6、PEDF、visfatin水平均显著降低(P<0.05),FSH、SHBG水平均显著升高(P<0.05),卵巢HE染色光镜下见囊性扩张的卵泡明显减少,黄体及各级发育期卵泡增多,卵巢颗粒细胞层数增厚。结论健脾化痰方对PCOS大鼠具有明显改善胰岛素抵抗及降低慢性炎症因子水平的作用,并能够改善卵巢功能、恢复排卵。
Objective：To establish a rat model of polycystic ovarian syndrome, and observe the effects of Invigorating Spleen and Removing Phlegm Decoction （ISRPD） on insulin resistance and serum adipokine levels in PCOS rats. Meth- ods：23- day old female SD rats were chosen and randomly divided into two groups, control group and experimental group. Rats in experimental group were injected daily with 6 mg/100 g weight of DHEA plus 0.2 mL oil for 20 days while rats in control group were injected with the same volume of oil. Then the PCOS rats were randomly divided into four groups,model group, metformin group, ISRPD group and metformin ＋ ISRPD group. After treated for 28 days, an IVGTT was applied to measure the levels of FBG, IVGTT - 2hBG, Fins, IVGTT - 2hIns. Sex hormone （ LH, FSH, T, SHBG） and adipokines（IL- 6, visfatin, PEDF）levels were also detected. Ovarian histological changes and ovulation condition of rats were examined with hematoxylin and eosin （HE） staining. Results ： Compared with control group, the levels of glucose and insulin were increased in model group. And compared with model group, the levels of glucose and insulin were decreased in all therapeutic groups. Compared with control group, the serum levels of T, LH, IL - 6 and PEDF were increased significantly in model group（P 〈0.05）. And compared with model group, the levels of T, LH, IL - 6, PEDF and visfatin were decreased significantly in all therapeutic groups （ P 〈 0.05 ） , and the levels of FSH and SH- BG were increased significantly in all therapeutic groups （ P 〈 0.05 ）. Ovarian hematoxylin and eosin stain showed that there were multiple follicular cysts on the ovaries of model group, and the number of granulose cells was decreased com- pared with control group. Kinds of developing follicles and corpus lutea were almost absent. In therapeutic groups, the number of follicular cysts was decreased compared with model group, and the numbers of developing follicles, corpus lut- ea and granulose cells were increased. Conclusion：Invigorating Spleen and Removing Phlegm Decoction can improve insu- lin resistance and decrease the serum levels of adipokines in PCOS rats. And it also can improve their ovulation.
出处
《中华中医药学刊》
CAS
2013年第11期2541-2544,I0012,共5页
Chinese Archives of Traditional Chinese Medicine
基金
南京市医学科技发展基金资助项目(ZKX11017)
