摘要
欧洲型猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)FJ0602和FJ0603株的ORF4～7基因进行了分段克隆、序列测定及同源性比较。结果显示,FJ0602和FJ0603株与LV核苷酸同源性为95.2%和95.3%,与VR2332核苷酸同源性为67.1%和66.9%,证实FJ0602和FJ0603株均为欧洲型PRRSV;与欧洲型弱毒疫苗株Amervac PRRSV有高度同源性,达到98.6%以上,提示分离毒PRRSV FJ0602和FJ0603株可能由疫苗株演化而来。FJ0602和FJ0603之间的核苷酸同源性为99.6%,表明这2毒株亲缘关系很近,很可能来源于同一毒株。
The ORF 4-7 genes for the PRRSV-FJ0602 and the PRRSV-FJ0603 were cloned, se- quenced and compared with those of other seventeen different PRRSV strains in GenBank by DNAstar software. The results showed that the nucleotide homology of ORF4-7 was 95.2 % and 95.3% with Lelystad virus,67.1% and 66.9% with VR2332,beyond 98.6% with the European type vaccine strain （Amervac PRRSV）, and 99.6% with each other,indicating that the PRRSV- FJ0602 and the PRRSV-FJ0603 belong to European type PRRSV and inherite from vaccine strain probably.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第11期1623-1626,共4页
Chinese Journal of Veterinary Science
基金
福建省畜牧科技重大专项(2006NZ003-2)
福建省科技计划项目(2008N0113)
关键词
猪繁殖与呼吸综合征病毒
欧洲型
ORF4
7
序列分析
porcine reproductive and respiratory syndrome virus
European type
ORF4-7
sequenceanalysis
