摘要
目的探讨KAI1/CD82 mRNA和CD82蛋白在鼻咽癌组织中的表达及其与临床病理特征的关系。方法采用免疫组化SP法检测70例鼻咽癌和30例正常鼻咽部组织中KAI1/CD82蛋白的表达情况;逆转录聚合酶链式反应(RTPCR)检测28例鼻咽癌和15例正常鼻咽部新鲜组织中KAI1/CD82 mRNA的表达情况。分析KAI1/CD82 mRNA和CD82蛋白的表达与临床病理特征之间的关系。结果 KAI1/CD82 mRNA在鼻咽癌组织中的阳性表达率为42.9%(12/28),低于正常鼻咽部组织中的73.3%(11/15),差异无统计学意义(P>0.05);KAI1/CD82蛋白在鼻咽癌组织中的阳性表达率为44.3%(31/70),低于正常鼻咽部组织中的70.0%(21/30),差异有统计学意义(P<0.05)。KAI1/CD82 mRNA和CD82蛋白在鼻咽癌组织中的表达与患者的性别、年龄、病理类型、T分期均无关(P>0.05),而与淋巴结转移有关。KAI1/CD82 mRNA和CD82蛋白在无淋巴结转移组中的阳性表达率分别为85.7%和68.4%,明显高于淋巴结转移组的28.6%和35.3%,差异均有统计学意义(P<0.05)。KAI1/CD82 mRNA和CD82蛋白在N1、N2、N3亚组间表达率的差异无统计学意义(P>0.05)。结论 KAI1/CD82 mRNA和CD82蛋白在鼻咽癌组织中表达下调,该基因可以抑制鼻咽癌发生、发展及淋巴结转移,有望作为鼻咽癌诊断和预后判定的一种有效分子标记物。
Objective To investigate the expression of KAI1/CD82 mRNA and CD82 protein in nasopharyngeal carcinoma,and the relationship of them with clinical features.Methods By immunohistochemical SP method,the CD82 protein expression was detected in 70 cases of nasopharyngeal carcinoma and 30 cases of normal nasopharyngeal tissues; By reverse transcription polymerase chain reaction （RT-PCR）,the KAI1/CD82 mRNA expression in 28 cases of nasopharyngeal carcinoma and 15 cases of normal nasopharyngeal fresh tissue were tested.The relationship of KAI1/CD82 mRNA and protein with clincopathological features were statistically analyzed.Results The positive expression rate of KAI1/CD82 mRNA in nasopharyngeal carcinoma was 42.9％ （12/28）,lower than 73.3％ （11/15） in the normal nasopharyngeal tissues（P ＞ 0.05）.The positive expression rate of CD82 protein in nasopharyngeal carcinoma was 44.3％ （31/70）,lower than 70.0％ （21/30） in normal nasopharyngeal tissues（P ＜ 0.05）.In nasopharyngeal carcinoma,the expression of KAI1/CD82 mRNA and CD82 protein was not related to the gender,age,histological type and T stage （P ＞0.05）,but related to lymph node metastasis （P ＜0.05）.The expression rates of KAI1/CD82 mRNA and CD82 protein in the group without lymph node metastasis were 85.7％ and 68.4％,were higher than 28.6％ and 35.3％ in the group with lymph node metastasis,and the difference were statistically significant （P ＜0.05）.The expression of KAI1/CD82 mRNA and CD82 protein in the subgroups of the stage N1-N3 had no significant difference（P ＞ 0.05）.Conclusion The down-regulation of KAI1/CD82 mRNA and CD82 protein expression is found in nasopharyngeal carcinoma.KAI1/CD82 can inhibit the process of nasopharyngeal carcinoma invasion and metastasis.So co-examination of KAI1/ CD82 mRNA and CD82 protein may be a biologic marker for diagnosis and prognosis of nasopharyngeal carcinoma.
出处
《临床肿瘤学杂志》
CAS
2013年第10期887-892,共6页
Chinese Clinical Oncology
关键词
鼻咽癌
转移抑制基因
KAI1
CD82
逆转录聚合酶链式反应
免疫组织化学法
Nasopharyngeal carcinoma
Metastasis suppressor gene
KAI1/CD82
Reverse transcription polymerase chain reaction （RT-PCR）
Immunohistochemistry
