摘要
酿酒酵母YMR034c基因与我们之前发现和鉴定的白念珠菌的CaRCH1基因同源,因此把它命名为ScRCH1,它编码的蛋白ScRch1在胞外高钙离子条件下定位于细胞质膜。为了研究酿酒酵母细胞对ScRch1质膜定位的调控机理,在编码细胞质膜和液泡膜等膜蛋白以及与脂质颗粒相关蛋白的402个基因的缺失株中,我们通过荧光显微镜技术检测了融合蛋白ScRch1-GFP的细胞膜定位情况。结果发现,ScRch1在其中10个基因的缺失株细胞里不能定位于质膜。这些基因包括两个编码细胞质膜运输蛋白的基因SNQ2和HXT1,一个编码液泡膜运输蛋白的基因AVT4,一个与液泡连接/融合相关的液泡膜蛋白基因PEP3,一个与细胞分化有关的内体膜蛋白基因DFG10,两个编码脂质体蛋白的基因EHT1和LDH1,以及三个功能未知的内体膜蛋白基因YBR219c、YBR224w和YDR417c。因此,ScRch1的细胞膜定位可能受到以上多个细胞过程的影响,这些研究结果为进一步阐明ScRch1的细胞质膜定位机制奠定了基础。
Saccharomyces cerevisiae YMR034c is a sequence homolog for the Candida albicans CaRCH1 gene, named as ScRCH1, and we show that ScRchl localizes to the plasma membrane in response to high levels of extracellular calcium. To find out membrane and lipid proteins related to the subcellular localization of ScRch 1, we screened 402 yeast single-gene deletion mutants for genes encoding membrane and lipid proteins through a fluores- cence microscope approach. We have identified 10 genes, whose deletion renders ScRchl-GFP failed to localize to the plasma membrane. These genes include SNQ2 and HXTI encoding plasma transport proteins, AVT4 encoding vacuolar transport proteins, PEP3 encoding docking/fusion tonoplast protein, DFGIO related to cell differentiation, EHTI and LDHI encoding liposome proteins, as well as YBR219c, YBR224w and YDR417c with unknown functions. These data provide a basis for our understanding the regulatory mechanisms of ScRchl subcellular localization.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2013年第11期1634-1642,共9页
Chinese Journal of Cell Biology
基金
江苏省高等教育机构重点学术研究开发计划
江南大学独立科学研究计划重点项目(批准号:JUSRP51313B)
国家自然科学基金(批准号:81371784
31301021)资助的课题~~
