摘要
选取40只雌性SD大鼠,分为4组,每组10只。除正常组(A组)外,模型组(B组)、干细胞移植组(C组)和仙灵骨葆组(D组)均灌胃维甲酸70 mg/(kg·d),连续灌胃14 d,A组用等量无菌水灌胃。B组注射生理盐水,C组尾静脉注射2×106脐带间充质干细胞,D组灌胃20 mg/kg仙灵骨葆。给药21 d后,测量各组大鼠血清钙离子和磷含量的变化以及ALP和StrACP水平,剥离大鼠右侧股骨并称量,双能X线骨密度仪测量右侧股骨骨密度,左侧骨切片进行电镜观察结构。结果表明,维甲酸诱导的大鼠血清钙离子、磷含量及ALP活性比正常组显著升高,C组和D组治疗后比模型B组有所下降;B组StrACP活性较A组高,C组与A组无显著性差异。B组骨湿重比正常A组显著减少,C组和D组骨湿重与A组无显著性差异;造模后B组骨密度显著下降,给药后C组和D组骨密度与A组无显著性差异;B组骨胶原纤维排列杂乱无序,结构松散并呈丝状,C组与B组相比,骨胶原纤维排列呈束状,走向一致,排列规则、紧密,恢复到与A组骨胶原相近形态和排列。结果提示,注射脐带间充质干细胞对改善维甲酸诱导的大鼠骨质疏松有一定的治疗效果。
To observe the therapeutic effect of umbilical cord mesenchymal stem cell on retinoicacid in- duced osteoporosis in female rats by intravenous injection, forty SD female rats were divided randomly into four groups： control group （Group A）, model group （Group B）, hUC-MSCs transplantation （Group C）, and Xianling Gubao group （Group D）. The rats in Group B, C and D were received retinoic acid at 70 mg/（kg-d） by gastric la- vage for 14 days to induce osteoporosis instead of sterile water in Group A. At the same time, Group C were adi- ministrated with 2 106 hUC-MSCs, Group D adiministrated with 20 mg/kg Xianling Gubao, and Group B were administrated with saline. After 21 days, the serum biochemical indicators, biomechanics characteristics were mea- sured and microstructure of bone were also observed. The results showed that the concentration of Ca2＋, Pi and ALP was increased appararently after RA induced, and it was recovered in Group B and C. The concentration of StrACP was increased in Group B and decreased in Group D vs Group A, but there was no difference in Group C. The wet weight of right femur was extremely decreased in Group B than Group A, and there was no difference statistically between Group C and D. The BMD was decreased after RA induced in Group B than Group A, and there was no difference between Group C and D. Examination of the collagenous fibers arrangement showed osseous collagen were deranged and surface loosening. Meanwhile, there was indication of repair in Group C. Taken together, these data suggested that RA-induced osteoporosis in rats could be relieved by injecting with umbilical cord mensenchy- real stem cells.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2013年第11期1592-1597,共6页
Chinese Journal of Cell Biology
基金
中央高校基本科研业务费专项资金(批准号:21612330)
广东省院士工作站建设项目(批准号:2012B090500007)
广东省科技计划项目(批准号:2011B040100019)资助的课题~~
关键词
脐带间充质干细胞
骨质疏松
细胞治疗
维甲酸
umbilical cord mesenchymal stem cells
osteoporosis
cell therapy
retinoic acid
