摘要
目的 :观察肝纤维化时 ,肝脏胶原类别变化与肝组织病理关系。方法 :通过免疫组化法检测不同肝纤维化时期鼠肝组织 ,结合电镜和常规病理等方法检测肝组织病理变化。结果 :肝纤维化早期Ⅰ型胶原变化不明显 ,Ⅲ、Ⅳ型胶原增多 ,以门脉区、肝细胞坏死区最为明显 ,而进入肝硬化活动期状态Ⅰ、Ⅲ、Ⅳ型胶原均增多 ,以Ⅰ型胶原增多为主 ,存在于纤维条索、肝窦、门脉区。电镜下显示 ,肝纤维化时 ,肝窦毛细血管基底膜呈连续状 (即肝窦毛细血管化形成 ) ,肝窦内胶原增生明显。结论 :Ⅰ、Ⅲ、Ⅳ型胶原在肝内沉积 ,肝窦基底膜破坏 ,肝纤维化时连续性基底膜的形成是慢性肝炎向肝硬化转变的病理基础。
Objective: To investigate the relationship of liver collagen types and pathologic histology that was anlysised in liver fibrosis. Methods:Types Ⅰ、Ⅲ、Ⅳ collagen in different periods of liver fibrosis were detected by using immunohistochemical method(PAP method), combining with methods of pathologic histology and electronic. Results: In the normal liver, type Ⅰ collagen appeaed around sinusoid, Ⅲ、Ⅳ collagen stayed in sinusoid except Ⅰ collagen area. In the early stage of hepatic fibrosis, the desposition of type Ⅲ、Ⅳ collagen but Ⅰ collagen increased significantly in hepatic injure. In liver cirrhosis, all of the types Ⅰ、Ⅲ、Ⅳ increased significantly and type Ⅰ collagen was the most. Electronic suggested the identifiable basement membranes in the space disse and defensrration of the endothelial cells socalled capillarization was developmented. Conclusion:The pathogensis of liver developmented with the development of hepatic fibrosis. The desposition of type Ⅰ、Ⅲ、Ⅳ collagen collelated significantly with the identifiable basement membranes in the space disse and defensrration of the endothelial cells so-called capilarization.
出处
《广州医药》
2000年第6期15-16,共2页
Guangzhou Medical Journal
关键词
肝纤维化
细胞外基质蛋白
免疫组织化学
Collagen type
Hepatic fibrosis
Immunohistochemistry
Hepatic sinusoidal capillarization
