摘要
采用细胞组织化学染色技术,以含笑发育各个时期的花药为材料,观察其发育中多糖和脂滴类物质的分布情况,探索其营养物质运输特征和规律。结果显示:(1)在花药中央的造孢细胞中有零星脂滴。在形成胼胝质壁的小孢子母细胞中脂滴数量增加,此时,在花药壁绒毡层细胞中出现少量脂滴,而在其他药壁细胞中则出现多糖颗粒,在四分体小孢子中依然有少量脂滴。(2)在游离小孢子早期,小孢子中的脂滴减少而出现多糖颗粒,此时,药室内壁细胞中积累的多糖颗粒消失,细胞径向伸长;在晚期小孢子内仍有较多的多糖颗粒和脂滴,此时,绒毡层细胞呈现出退化,其中出现一些脂滴。(3)在早期二胞花粉中,液泡逐渐消失,多糖颗粒明显增加;在成熟花粉中,脂滴的数量有所减少,仍保持较多的多糖颗粒作为花粉储存物。研究认为,含笑小孢子在母细胞时期的绒毡层细胞内有少量的脂滴,没有淀粉多糖,说明绒毡层细胞活跃地将营养物质转运到药室中,故呈现较少脂滴的现象,或是含笑绒毡层细胞并没有转化糖类为脂类的功能,仅仅起了将中层细胞中的脂滴转运到药室中的作用。
Using cytochemical technique,we observed the distribution of polysaccharides and lipids during the anther development of Michelia figo：（1）A few lipids were accumulated in sporogenous cells.At microspore mother cell（MMC）stage,a few lipids were still located in the MMCs which had formed a callose wall.At the same time,a few lipids were accumulated in tapetal cells,but many polysaccharides appeared in other cells of anther wall.After MMC meiosis,tetrad microspores still had a few lipids.（2）After released from tetrad microspores,the free microspores began to accumulate polysaccharides and the number of lipids decreased.At this time,polysaccharides in anther wall cells decreased and endothecium cells enlarged from radial direction.In late microspores,there were still many polysaccharides and a few lipids in the cells.Tapetal cells began to degenerate at this time and many lipids appeared in the cells.（3）In early bicellular pollen,the vacuoles disappeared,and the number of polysaccharides increased evidently.In nearly mature pollen of M.figo,though the lipids had been less,there were still many polysaccharides and some lipids accumulated in the vegetative cells as the storage material of pollen.The study suggested that tapetal cells at microspore mother cell stage contained little number of lipids and no polysaccharides.It appeared that tapetal cells transported nutriment to chamber actively,or transported lipids from middle layer cells without converting to polysaccharides.
出处
《西北植物学报》
CAS
CSCD
北大核心
2013年第10期1981-1985,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然基金地区科学基金项目(41361095)
关键词
含笑
花药发育
多糖
脂滴
Michelia figo
anther development
polysaccharides
lipid
