摘要
从7日龄北京油鸡胚胎肝脏中分离间充质干细胞,原代培养并传代至15代,免疫荧光法检测造血祖细胞/肝卵圆细胞的表面标志物CD34、CK19呈阴性表达;RT-PCR检测CD29、CD44、CD71、CD73呈阳性表达。尝试不同培养条件对细胞生长曲线的影响,选取最佳培养方案,同时对细胞进行细胞周期测定。肝脏间充质干细胞通过不同诱导液被成功诱导分化成脂肪细胞、心肌细胞,结果表明,从鸡胎肝中分离获得的间充质干细胞具有和人类间充质干细胞相似的生物学特性及分化潜能,其所具有多向分化的潜能,为今后临床广泛应用提供了可能。
Mesenchymal stem cells （MSCs） from chicken fetal liver are multipotent stem cells that can differentiate in vitro into various terminally differentiated cells. The majority of studies of MSCs have been focused on human. In this study, chicken liver-derived MSCs were isolated from 7-day-old em- bryo of Beijing fatty chickens. Primary liver-derived MSCs were sub-cultured to passage 15. The surface markers,CD29,CD44,CD71 and CD73 of liver-derived MSCs were detected by RT-PCR and the surface markers,CD34 and CK19 of hematopoietic progenitor cells/hepatic oval cells were not detected by im- munofluorescence. The best culture conditions were determined by drawing growth curve and the cells cycle were investigated. Liver-derived MSCs were successfully induced and differentiated into adipocytes and myocardial cells. The results suggested that the MSCs isolated from chicken fetal liver possess simi- lar biological characteristics with those derived from human,and their multilineage potential would pro- vide many possible applications.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2013年第6期99-105,共7页
Journal of Huazhong Agricultural University
基金
转基因生物新品种培育科技重大专项(2011ZX08009-003-006
2011ZX08012-002-06)
基本科研业务项目(2012z1072)
国家家养动物种质资源平台项目(2012)
关键词
北京油鸡
间充质干细胞
生长曲线
诱导分化
Beijing fatty chickenl mesenchymal stem cellsl growth curvel differentiation potential
