摘要
目的 探讨荧光定量聚合酶链反应（FQ-PCR）检测人乳头瘤病毒（HPV）在宫颈癌筛查中的应用价值.方法 采用FQ-PCR法对妇科门诊1 590例患者进行8种高危型HPV检测,同时取病变宫颈组织进行病理组织活检,分析两者的关系.结果 1 590例患者中HPV-DNA阳性438例,阳性率27.5%,各种病理类型的宫颈病变中HPV感染率分别为：慢性炎症75.1%（329/438）,癌前病变22.4%（98/438）,宫颈癌2.5%（11/438）;523例疑似病例中HPV-DNA阳性331例,各种病理类型的宫颈病变中HPV感染率分别为：慢性炎症50.1%（194/387）,癌前病变90.6%（106/117）,宫颈癌100%,差异具有统计学意义（P＜0.05）.结论 荧光定量聚合酶链反应（FQ-PCR）检测高危型HPV在宫颈癌筛查中具有重要作用.
Objective To evaluate the value of fluorescence quantitative polymerase chain reaction （FQ-PCR） detecting HPV （Human papillomavirus） in screening of cervical cancer. Methods The investigation was carried out in 1 590 female outpa-tients in hospitals. The cervical secretion were detected for eight kinds of high-risk HPV by FQ-PCR. Meanwhile analyzed the relationship between HPV-positive rate and the pathological type. Results In the 1 590 patients,there 438 patients posi-tive for HPV-DNA were detected with an incidence of 27. 50%. The infection rates of HPV in chronic cervicitis, precancerous change and cervical cancer were 75.1%, 22.4% and 2.5%. In the 523 suspicious patients, there 331 patients positive for HPV-DNA were detected. The infection rates of HPV in chronic cervicitis, precancerous change and cervical cancer were 50. 1%, 90. 6% and 100%. There was a significant difference between the rate of HPV infection and dfferent severity cervi-cal diseases （P〈0. 05）. Conclusion The fluorescence quantitative polymerase chain reaction （FQ-PCR） detecting high-risk HPV has an important role for screening of cervical cancer.
出处
《现代检验医学杂志》
CAS
2013年第5期121-122,125,共3页
Journal of Modern Laboratory Medicine
关键词
荧光定量聚合酶链反应(FQ-PCR)
宫颈癌
筛查
fluorescence quantitative polymerase chain reaction（FQ-PCR）
cervical cancer
screening
