摘要
目的探讨实验性大鼠蛛网膜下腔出血(subarachnoid hemorrhage,SAH)后外周血白细胞计数的变化趋势和MMP-9/TIMP-1比值的变化,及其与早期脑损伤(early brain injury,EBI)的相关性。方法用大鼠枕大池单次注血法建立SAH动物模型,行外周血白细胞计数,ELISA法检测外周血MMP-9和TIMP-1浓度,干湿重法测量脑组织水含量,并行基底动脉HE染色,测量管腔内径变化。结果 SAH后6h脑组织水含量明显升高,24h时达到最高值,白细胞计数、外周血MMP-9含量均在24h时达到最高,基底动脉内径在12h时明显变化,24h时达到最低值,72h基本恢复正常。相关性研究发现,白细胞计数和MMP-9/TIMP-1比值的变化与基底动脉痉挛和脑组织含水量具有显著的相关性。结论 SAH后早期外周血白细胞计数和MMP-9/TIMP-1比值呈明显的动态变化,此变化趋势与早期脑损伤相关指标间有显著的相关性;外周血MMP-9/TIMP-1比值可以作为判断SAH后EBI的指标。
Objective To investigate the trends of peripheral blood leucocyte counts and MMP-9/TIMP-1 ratio in acute experimental subarachnoid hemorrhage model in rats and their relationship with early brain injury. Methods A rat single cisterna magna injection model was used. Leukocytes were counted by blood counting chamber, and MMP-9 and TIMP-1 were measured by ELISA. The dry-wet weight method was used to measure brain water content. The inside diameter was measured on an HE stained basilar artery. Results The brain water content increased significantly at 6 h after subarachnoid hemorrhage and peaked at 24 h, so did the leukocyte count as well as MMP-9 and TIMP-1 contents. Both the leukocyte count and MMP-9/TIMP-1 ratio were related to early brain injury after subarachnoid hemorrhage. Conclusion The leukocyte count and MMP-9/TIMP-1 ratio increase significantly in the early stage of subarachnoid hemorrhage and return to normal after 72 h. The trend is related to early brain injury after subarachnoid hemorrhage. The MMP-9/TIMP-1 ratio may be used as a predictor of the appearance of early brain injury.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2013年第6期717-721,727,共6页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家自然科学基金资助项目(No.30870844)
陕西省"13115"重大科技创新专项基金资助项目(No.2008ZDKG-66)
教育部高等学校博士学科点专项科研基金(No.20110201110060)~~
