摘要
背景:异丙肾上腺素注射能够促进啮齿类动物涎腺腺泡细胞增殖、肥大。然而,异丙肾上腺素注射后涎腺干/祖细胞的增殖能力目前仍不清楚。目的:研究异丙肾上腺素腹腔注射对SD大鼠下颌下腺干/祖细胞激活、增殖能力。方法:SD大鼠随机分为2组,分别腹腔注射异丙肾上腺素与生理盐水,连续注射5 d后取下颌下腺,酶消化法制取下颌下腺细胞悬液,体外培养,获得下颌下腺类上皮细胞集落并计数。挑取增殖较快的集落,进行CD90.1、层粘连蛋白、α6β1等免疫细胞化学检测。结果与结论:与对照组相比,异丙肾上腺素注射组中、低增殖集落数量较少,高增殖集落数量较多,差异有显著性意义(P<0.05),而集落总数无明显区别(P>0.05)。高增殖下颌下腺类上皮细胞集落CD90.1、层粘连蛋白、α6β1抗体均为阳性表达。实验结果显示,异丙肾上腺素注射不能明显增加下颌下腺中的干/祖细胞数量,但是能提高腺体中干/祖细胞的增殖能力。
BACKGROUND：Injection of isoproterenol is known to induce proliferation and hypertrophy of acinar cells in rodent salivary glands. However, the clonal proliferation ability of stem/progenitor cells of salivary glands by isoproterenol remains unclear.
OBJECTIVE：To study the proliferation and activation ability of stem/progenitor cells of submandibular gland with colony assay by intraperitoneal injection of isoproterenol.
METHODS：Sprague-Dawley rats were randomly divided into two groups, isoproterenol and control groups, respectively intraperitonal y injected with isoproterenol and normal saline for 5 consecutive days. The gland tissues were harvested, and the stem/progenitor cells of submandibular gland were obtained by enzyme digestion in vitro. The number of clonal colonies of each group was analyzed. The larger colony cells were col ected for immunohistochemistry staining with CD90.1, laminin andα6β1.
RESULTS AND CONCLUSION：The number of middle and low proliferative potential colony-forming cells was less but high proliferative potential colony forming cells were significantly more in isoproterenol group compared with control group （P〈0.05）. However, there was no significant difference in the total number of the colonies between two groups （P〉0.05）. The high proliferative potential colony forming cells were positive for CD90.1, laminin andα6β1. Results showed that isoproterenol treatment model cannot increase the cellnumber, but enhance the proliferation ability of stem/progenitor cells from the submandibular gland.
出处
《中国组织工程研究》
CAS
CSCD
2013年第40期7084-7089,共6页
Chinese Journal of Tissue Engineering Research
基金
贵州省科技厅科技攻关项目基金(SY[2011]3016)
贵州省科学技术基金(黔科合J字LKZ[2011]23)~~
