摘要
以白肉火龙果果皮为原料,采用纤维素酶法提取其原花青素,以原花青素提取液吸光度为检测值,分别对酶体积分数、酶解温度和乙醇浓度进行单因素试验,并在此基础上采用Box-Behnken中心组合试验设计及响应面分析,建立回归模型。结果表明:酶法提取火龙果皮中原花青素的最佳工艺为酶质量分数0.95%、酶解温度30℃、乙醇浓度60%。
Cellulase was used to extract proanthocyanidins from fruit pericarp of pitaya （Hylocereus undatus）. Based on results of single-factor experiments, Box-Behnken central composite design coupled with response surface methodology involving there factors, enzyme concentration, extraction temperature and ethanol concentration, was applied to set up a quadratic polynomial regression equation for proanthocyanidins extraction. The optimal extraction conditions of proanthocyanidins from pitaya pericarp were as follows： cellulase dosage 0.95%, extraction temperature 30℃ and ethanol concentration 60%.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第20期97-100,共4页
Guangdong Agricultural Sciences
基金
广东省高等院校学科建设专项(2008-342)
广东省2012年度大学生创新训练项目(1107812065)
广州市属高校科研计划项目(2012A047)
关键词
火龙果
原花青素
纤维素酶
提取
响应曲面法
pitaya
proanthocyanidins
cellulase
extraction
response surface methodology
