摘要
目的:观察绞股蓝总皂苷(gypenosides)对宫颈癌HeLa细胞的生长抑制作用,并初步探讨其可能的分子机制。方法:分别采用不同质量浓度的绞股蓝总皂[4.5μg/mL(低剂量组)、45μg/mL(中等剂量组)和450μg/mL(高剂量)]处理HeLa细胞,以0.9%氯化钠溶液处理的HeLa细胞作为对照组。分别采用MTT法和5-溴脱氧尿嘧啶核苷(5-bromo-2-deoxyuridine,BrdU)掺入法观察绞股蓝总皂苷对宫颈癌HeLa细胞的生长抑制作用;FCM法观察绞股蓝总皂苷对细胞凋亡的影响;蛋白质印迹法检测绞股蓝总皂苷对Bcl-2、Bax、细胞外调节蛋白激酶1/2(extracellular regulated protein kinases1/2,ERK1/2)、磷酸化ERK1/2(phospho-ERK1/2,p-ERK1/2)、丝裂原活化蛋白激酶激酶(mitogen-activate protein kinase kinase,MEK1/2)和p-MEK1/2蛋白表达的影响。结果:绞股蓝总皂苷质量浓度45μg/mL处理HeLa细胞24 h后细胞存活率显著降低(P<0.01),4.5μg/mL处理48 h后也能够显著降低HeLa细胞的存活率(P<0.01);BrdU掺入法检测结果提示,绞股蓝总皂苷质量浓度450μg/mL时能够抑制HeLa细胞的增殖。不同剂量的绞股蓝总皂苷处理HeLa细胞后,凋亡率均显著增加;Bcl-2和p-ERK1/2的表达下调,而Bax表达上调。结论:绞股蓝总皂苷对HeLa细胞具有凋亡诱导和增殖抑制作用,其机制可能与Bax表达上调以及Bcl-2、p-ERK1/2表达下调有关。
Objective： To investigate the inhibitory effect of gypenosides on proliferation of human uterine cervical cancer HeLa cells in vitro, and to explore its possible mechanism. Methods： The HeLa cells were treated with different concentrations of gypenosides （low-dose group, 4.5 I^g/mL; middle- dose group, 45 I^g/mL; high-dose group, 450 μg/mL）. The cells treated with 0.9% sodium chloride solution was designed as a control group. The inhibitory effect of gypenosides on the proliferation of HeLa cells was detected by MTT assay and BrdU （5-bromo-2-deoxyuridine） incorporation experiment. The effect of gypenosides on apoptosis of HeLa cells was detected by FCM （flow cytometry）. The expression levels of Bcl-2, Bax, ERK1/2 （extracellular regulated protein kinase 1/2）, p-ERK1/2 （phospho-ERK1/2）, MEK1/2 （mitogen-activated protein kinase kinase） and p-MEK1/2 were detected by Western blotting. Results： The survival rate of HeLa cells was decreased significantly after treatment with gypenosides （45 i,g/mL） at 24 h （P 〈 0.01）. The survival rate of HeLa cells was decreased significantly after treatment with gypenosides （4.5 μg/mL） at 48 h （P 〈 0.01）. The inhibitory effect of gypenosides （450 μg/mL） on the cell proliferation was confirmed by BrdU incorporation experiment. The apoptotic rate of HeLa cells was increased significantly after treatment with different concentrations of gypenosides. Gypenosides could down-regulate the expression levels of Bcl-2 and p-ERK1/2 and up- regulate the expression level of Bax. Conclusion： Gypenosides can significantly inhibit the proliferation of cervical cancer HeLa cells and also markedly induce the apoptosis. This effect may related to the up- regulation of Bax expression and down-regulation of Bcl-2 and p-ERK1/2 expressions.
出处
《肿瘤》
CAS
CSCD
北大核心
2013年第10期868-872,共5页
Tumor
