摘要
目的探讨表没食子儿茶素没食子酸酯(EGCG)抑制鼻咽癌干细胞SP18增殖的机制。方法采用MTT法检测EGCG抑制SP18细胞增殖的IC50;选用细胞生长曲线、平皿克隆形成实验和软琼脂集落形成实验观察EGCG对鼻咽癌干细胞SP18增殖的影响;采用Western blot检测Notch1和Jagged1蛋白表达。结果 EGCG抑制SP18细胞增殖的IC50值为149.02 mg/L;EGCG呈浓度和时间依赖性抑制SP18细胞的增殖(n=3,P<0.05);EGCG呈浓度依赖性抑制Notch1和Jagged1蛋白的表达。结论 EGCG能有效抑制鼻咽癌干细胞SP18的增殖,其可能通过降低Notch1和Jagged1蛋白的表达而发挥作用。
Objective To analyze the mechanism of Epigallocatechin-3-gallate （EGCG） inhibiting the proliferation of nasopbaryngeal cancer stem cell SP18. Methods 50% inhibiting concentration （ ICs0 ） of which EGCG inhibited the proliferation of nasopharyngeal cancer stem cell SP18 was detected by MTT method. The proliferation of SP18 cells affected by EGCG was analyzed by the cellular growth curve, colony formation and soft agar formation. Then the expression of Notehl and Jaggedl protein were respectively detected by Western blot in SP18 cells treated by EGCG. Results IC50, of which EGCG inbibted the proliferation of SP18 cells ,was 149.02 mg/L. The proliferation of SP18 cells was inhibited with EGCG concentration increasing and treatment time extending （ n = 3 ,P 〈 0.05 ）. The expression of Notchl and Jaggedl proteins in SP18 cells was obviously deceased with EGCG concentration increased. Conclusion EGCG can inhibit the proliferation of nasopharyngeal cancer stem cell SP18, which probably decreased the expression of Notchl and Jaggedl proteins to a- chieve the role.
出处
《中南医学科学杂志》
CAS
2013年第5期440-444,共5页
Medical Science Journal of Central South China
基金
湖南省自然科学省市联合基金(12JJ9033)
湖南省自然科学基金(12JJ3102
13JJ3079)
湖南省高校创新平台开放基金(12K094
10K052)
湖南省研究生科研创新项目(CX2010B380)
湖南省教育厅科研项目(11C1112
12C0340)
衡阳市科技局科研项目(2011kj52
2011kj53)
