摘要
采用粘平端定向基因克隆技术 ,成功地构建了携带HSAcDNA基因片段的重组逆转录病毒质粒载体GINHSAa。经限制性酶切鉴定和菌落原位杂交证明HSAcDNA已克隆到G1Na当中并得到
In this article,GINHSAa,a recombination plasmid using new retroviral vector imediated cDNA of HSA was successfully constructed by applying for flush stick and linkage method.It proved by both the restriction endonuclease and bacteria in situ hybridization that cDNA of HSA had already been linked into MCS of the G1Na vector end 9 positive clones were achieved.
出处
《湖北农业科学》
2000年第5期57-59,共3页
Hubei Agricultural Sciences
基金
湖北省农业科学院和畜牧兽医研究所自选课题资助
