摘要
以3-羟基丁酸-co-3-羟基戊酸共聚物(PHBV)为唯一碳源培养PHBV降解菌DS04-T,考察培养时间、温度、培养基起始pH、摇床转速、接种体积分数以及装液量等环境因素对菌株产生PHBV降解酶的影响,进而考察菌株对PHBV的降解能力。并结合正交试验优选适宜的产酶条件如下:培养温度28℃;培养时间32h;摇床转速150r/min;装液量100mL培养基;培养基起始pH为7.5;接种体积分数1.5%。在此优化条件下菌株产生的PHBV降解酶有较高的活力,达(18.9±1.2)U/mL。
Using poly（3-hydroxybutyrateco 3-hydroxyvalerate） （PHBV） as sole carbon source, the biodegradation o{ the PHBV by Pseudomonas mendocina DS04-T was studied. The effect of incubation time, cultivation temperature, initial pH, shaking revolution, liquid medium volume and inoculation content on PHBV degradability were studied. According to the results of orthogona] test, the optima] conditions of enzyme production were chosen as follows： temperature 28 ℃, incubation time 32 h, initial pH 7.5, shaking revolution 150 r/min, inoculation content 1.5% , and 100 mL culture medium is load in 250 mL flask. Under the optimum condkions, the PHBV-degrading enzymatic activity reached （18.9± 1.2） U/mL.
出处
《辽宁石油化工大学学报》
CAS
2013年第3期4-7,共4页
Journal of Liaoning Petrochemical University
基金
国家自然科学基金项目(31100099)
辽宁省教育厅科学研究项目(L2011060)
