摘要
以榆黄蘑原种、栽培种为试材，采用摇瓶培养榆黄蘑液体菌种，通过单因子试验筛选较优的速效碳源和迟效碳源，较优的复合碳源比、碳氮比、培养时间，并通过回管法进行菌种落发试验。结果表明：较优的速效碳源和迟效碳源分别为麦芽糊精和玉米淀粉，较优的速效碳源和迟效碳源比例为1：2，较优的碳氮比为12：1，最适培养时间为5d，采用此条件制备的液体菌种栽培榆黄蘑，菌丝满袋时间较固体菌种减少1～2d，生物学效率与固体接种相近。
Taking original species and cultivars of Pleurotus citrinopileatus as test materials, using shake flasks citrinopileatus liquid spawn, the more fast-acting and slow-acting carbon source ratio, complex carbon source ratio, carbon- nitrogen ratio,culture time were screened by single factor experiment, and strain germination experiment was done by return pipe method. The results showed that the best more fast-acting and slow-acting carbon carbon were maltodextrin and corn starch. Acting on better carbon and delayed-carbon ratio was 1 -2, and more of the carbon-nitrogen ratio of 12 ： 1 ;optimal culture time was 5 d;using this condition citrinopileatus liquid spawn cultivation,bacteria solid silk bag full of bacteria decreased over 1-2 d, biological efficiency and solid vaccination similar.
出处
《北方园艺》
CAS
北大核心
2013年第20期131-134,共4页
Northern Horticulture
基金
广西科学研究与技术开发计划资助项目(桂科能1346007-26)
南宁市科学研究与技术开发计划资助项目(20122083)
关键词
榆黄蘑
液体菌种
碳源
Pleurotus citrinopileatus
liquid strain
carbon source
