摘要
目的建立RP—HPLC测定鱼腥草中芦丁和槲皮素含量的方法。方法采用色谱柱：ODS—C18柱（150x4．6ram，5μm），流动相：甲醇-1％乙酸水（55：45，v／v）梯度洗脱．流速：1．0mL·min-1，检测波长：260nm，枉温：30~C。结果芦丁进样浓度在6．0—144．Oμg·mL-1。范围内线性关系良好，r=0．9984，平均回收率为97．4％（RSD=1．38％）；槲皮苷进样浓度在8．0～128．0μg·mL-1。范国内有良好的线性关系，r=O．9995，平均回收率为93．3％（RSD=1．82％）；槲皮素进样浓度在4．0~96．0μ·mL-1范围内有良好的线性关系，r=0．9995．平均回收率为87．3％（RSD=1．58％）。结论谊方法简便、准确度较高、重现性好，为鱼腥草中药材的质量控制提供了实验依据。
OBJECTIVE To establish a RP-HPLC method for determination of rutin and quercetin in Houttuyn- ia cordata Thunb method. METHODS Chromatographic column was used：0DS-Cls column （ 150 x 4. 6mm,5 μm） ,mobile phase of methanol： water （ 55： 45, -1% acetic acid V/V） gradient elution, flow rate： 1.0mL ~ min - 1, detection wavelength：260nm,column temperature：30~C. RESULTS Rutin sample concentration in 6.0 ~ 144. 01~g ~ mL-1 showed a good linear relationship, r = 0. 9984, the average recovery rate was 97.4% （ RSD = 1.38% ） ; quercitrin sample concentration in the range of 8.0 ~ 128.0~g ~ mL-I has a good linear relationship, r = 0. 9995, the average recovery rate was 93.3% （ RSD = 1.82% ） ; Quercetin sample concentration in the range ofg. 0 ~96. 0~g ~ mL-1 has a good linear relationship, r = 0. 9995, the average recovery rate was 87.3% （RSD = 1.58% ）. CONCLUSION The method is simple, accurate, reproducible provides the experimental basis and for the quality control of Chinese medicinal materials in Houttuynia cordata Thunb.
出处
《海峡药学》
2013年第9期93-95,共3页
Strait Pharmaceutical Journal
