摘要
以盐溶蛋白质质量分数、巯基质量摩尔浓度、Ca2+-ATPase活性为指标,考察草鱼在3种储藏方法:A,-60℃冻结24h后-20℃冻藏;B,-40℃冻结24h后-20℃冻藏;C,-20℃直接冻结,处理下冻藏及随后4℃冷藏期间肌肉蛋白质生化特性的变化情况。结果表明:不同冻结方法草鱼盐溶性蛋白质质量分数、巯基质量摩尔浓度以及Ca2+-ATPase活性随着贮藏时间的延长均呈下降趋势。冻藏前6周,A处理的盐溶蛋白质质量分数显著高于C处理(P<0.05),而B和C没有显著性差异(P>0.05);在前4周,A处理的巯基质量摩尔浓度显著高于C处理(P<0.05),A和B、B和C间没有显著性差异(P>0.05);Ca2+-ATPase活性在第2周迅速下降并一直处于较低水平,A、B和C三者间没有显著差异(P>0.05)。在冷藏期间,盐溶蛋白质质量分数、巯基质量摩尔浓度继续下降,Ca2+-ATPase活性虽有波动但活性仍然很低。从本试验可以看出,冻藏前的低温冻结处理对草鱼冻藏蛋白质盐溶性和巯基含量影响较大,能够降低蛋白质变性程度,并且温度越低,蛋白质变性程度越小,而对其Ca2+-ATPase活性的变化影响不明显。
The study investigated the biochemical properties of muscle protein from grass carp by evaluating the content of salt soluble protein and sulfhydryl group, Ca^2＋-ATPase activity when the grass carp experienced three freezing methods ;A, - 60 ℃ for 24 h then - 20 ℃ frozen storage;B, - 40 ℃ for 24 h then - 20 ℃ frozen storage℃, -20 ℃ frozen storage. Results showed that the content of salt soluble protein and sulfhydryl group, Ca^2＋ -ATPase activity of fillets treated by different freezing methods decreased as time. During frozen storage, the content of salt soluble protein for A treatment was significantly higher than that of C treatment（P〈0.05） before 6 weeks, and there was no significant difference between B and C treatments（P〉0.05）. The content of sulfhydryl group for A group was significantly higher than that of C group（P〈0.05） before 4 weeks, and no significant differences were found between A and B, B and C groups（P〈0.05）. Ca^2 ＋ -ATPase activity declined quickly at 2rid week and remained a low level. During chilling storage,the content of salt soluble protein and sulfhydryl group were declining, and the Ca^2＋ -ATPase activity fluctuated but was still at a very low value. So low temperature freezing treatment before frozen storage had remarkable effect on solubility of actomyosin and sulfhydryl content, except for Ca^2＋ -ATPase activity, which could reduce the protein denaturation,and the lower temperature the better effectiveness.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2013年第6期158-163,共6页
Journal of China Agricultural University
基金
现代农业产业技术体系建设专项资金资助(CARS-46)
关键词
草鱼
冻结方法
肌原纤维蛋白
冷冻变性
freezing treatments
grass carp fillets
myofibrillar protein
frozen denaturation
