摘要
为研究甜瓜属种间杂交质体DNA的遗传规律,利用PCR直接测序法对甜瓜属异缘四倍体新种(Cucumis×hytivus Chen and Kirkbride.)S5自交后代及其杂交母本甜瓜属野生种(Cucumis hystrix Chakr.)和父本栽培黄瓜‘北京截头’(Cucumis sativus cv.‘Beijingjietou’)叶绿体基因Matk-trnK和rbcL-accD区域的部分DNA序列进行比较分析。结果显示在长度为2 036bp Matk-trnK区域中存在着18个多态性位点,其中有16个多态性位点的碱基子代与母本相同,只有2个位点与父本相同;在长度为945bp的rbcL-accD区域中存在着19个多态性位点,其中有18个多态性位点的碱基子代与母本相同,只有1个多态性位点与父本相同。这表明甜瓜属hystrix×sativus种间杂交中质体DNA遗传主要表现为母系遗传。
To investigate the inheritance of plastid DNA in the interspecific crossing of Genus Cucumis, the DNA sequences in partial region of chloroplast Matk-trnK and rbcL-accD gene from cucumis allotetraploid （Cucumis × hytivus Chen and Kirkbride. ） S5 selfing progeny, cultivated cucumber ＇ BeijingJietou＇ （ C. sativus L. ） the paternal parents and the wild Cucumis species（ C. hystrix Chakr. ） the maternal parents, were comparatively analysed, using PCR sequencing method. Eighteen polymorphic loci were detected in 2 036 bp length Matk-tmK gene region, sixteen polymorphic loci in allotetroploid as the its maternal parents,two as its paternal parents. Nineteen polymorphic loci were detected in 945 bp length rbcL-accD gene region, eighteen polymorphic loci in allotetraploid as its maternal parents, only one as its paternal parents. The result demonstrated that plastid genomes was maternal inheritance pattern in hystrix x sativusin species crosssing among Genus Cucumis.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2013年第6期143-147,共5页
Journal of China Agricultural University
基金
国家自然科学基金重点项目(30830079)
教育部‘111’项目(B08025)
江苏省三项工程项目(SXGC(2012)373)
江苏省青蓝工程项目资助
关键词
甜瓜
质体
多态性位点
母系遗传
Cucumis
plastid
polymorphic Ioci
maternal inheritance
