摘要
本文以整体低渗的方法进行哺乳类与鸟类的染色体制备,以探讨一种在野外条件下,无法进行离心时,适合细胞遗传学研究的染色体制备新方法。应用该方法可在野外考察中,同时开展细胞遗传学方面的工作在某些特殊研究课题中,该方法有着广阔的应用前景。
We describe a new feasible chromosome procedure detailed as follows:
1. Take out of the femur or tibia from birds and small mammals, and sternum or rib from large mammals.
2. Scissor it carefully and treat for 50 minutes with hypotonic solution.
3. Squeeze it, and fix with Carnoy's solution.
4. Prepare the slide by air-drying method.
We have obtained quite a few good chromosome specimens by this procedure. About 66% of the birds' and almost all of the mammals' chromosome prepar-tions are succeed in our experiments by this technique. Using this method we can begin cylogenctic works in some poor conditions e. g. when centrifuge or electricity is not available. This technique may be employed in some special research programs as well as in field investigations.
