摘要
目的研究原发性肝细胞癌(HCC)组织中p21的亚细胞定位及其意义。方法收集115例肝硬化基础上发生的肝癌病理标本,包括同一病例的肝癌组织、癌旁组织、肝硬化组织,分为肝癌组、癌旁组、肝硬化组,采用S-P法分别行p21的免疫组织化学染色,并观察p21的亚细胞分布,与肝癌的临床病理特征、癌细胞分化程度进行相关性分析。结果 HCC组和癌旁组均有p21的高表达(35.7%,38.3%),两者之间的差异无统计学意义(P>0.01),明显高于肝硬化组(10.4%,P<0.01)。p21核内表达的阳性率,肝硬化组与癌旁组相似(91.7%,93.2%,P>0.01),明显高于肝癌组(24.4%,P<0.01),p21细胞质表达的阳性率最高(75.6%,P<0.01)。p21的亚细胞定位分布与肝癌患者的发病年龄、乙肝病毒的感染、肿瘤大小无明显相关性(P>0.01或>0.05),但与肿瘤细胞的分化程度、肿瘤转移具有良好的相关性;低分化、伴有肿瘤转移的肝癌患者,其p21的细胞质表达率更高。结论在肝硬化向肝癌转化的过程中,伴有p21亚细胞定位的改变,即由胞核移位到细胞质,且肿瘤细胞恶性程度愈高,肿瘤转移愈早,细胞质移位比例愈高。且这种亚细胞定位分布的改变,与肿瘤患者的发病年龄、乙肝病毒的感染、肿瘤大小无关。
Objective To investigate the subcellular localization of p21 in hepatocellular carcinoma （HCC） tissues and its clinical significance. Methods 115 cases of HCC tissues based on liver cirrhosis were performed with immunohistoehemical staining and subcellular localization for p21 was observed. The correlation between subcellular localization of p21 and patients＇ clinicopathological parameters was analyzed. Results The positive rate of p21 in HCC tissues was same with that in paracancerous tissues（35.7% vs 38.3% ,P 〉0. O1 ） ,and significantly higer than that in liver tissues of cirrhosis（ 10.4% ,P 〈0.01 ）. p21 was mainly localized in nucleus in cirrhosis and paracancerous tissues （91.7% vs 93.2% ,P 〉 0.01 ） ,but the positive rate of p21 in cytoplasm was the highest in HCC tissues （75.6% P 〈 0.01 ）. There was a significant correlation between the localization of p21 in cytoplasm and the tumor differentiation or metastasis, and the localization of p21 was not related with the age, HBV infection, size of the tumor; but with the differentiation and metastasis of the tumor. Conclusions io21 may change its subcellular localization in the process of transformation from cirrhosis to tumor. Higher the degree of malignancy of the tumor cells or earlier the tumor metastasis is, larger the preportion of cytoplasmic shift has. And the distribution of this subcellular localization has no association with the age of onset of tumors, size of the tumor or hepatitis B virus infection.
出处
《实用肿瘤杂志》
CAS
2013年第5期488-491,共4页
Journal of Practical Oncology
基金
湖南省科技厅项目(No.2012FJ3118)
关键词
肝肿瘤
P21
肝硬化
肿瘤转移
免疫组织化学
liver neoplasms
p21
liver cirrhosis
neoplasm metastasis
immunohistochemistry
