摘要
目的 :为了探讨中性粒细胞 (PMN)的自然凋亡和PMN凋亡对细胞吞噬、趋化和活性氧 (ROS)生成等功能的影响。方法 :采用光镜、电镜、电泳和流式细胞仪等研究手段探讨PMN的自然凋亡 ;通过葡萄球菌法、滤膜法和化学发光的方法研究凋亡PMN吞噬、趋化和ROS生成的改变。结果 :分离健康成人外周血PMN ,在体外进行 10 %血清培养 ,随着培养时间的延长 ,PMN凋亡率升高 ,同时PMN的吞噬、趋化以及ROS生成等功能都不同程度地受损。结论 :外周血PMN在体外培养发生自然凋亡 ,PMN的凋亡与细胞功能相关。
Objective: To understand the apoptosis of PMN in the absence of stimuli. To study the phagocytosis of apoptotic neutrophils,its ability to migrate toward a chemoattractant, and also its ability of superoxide releasing. Methods: PMN apoptosis was determined by light microscopy,electron-microscopy,gel electrophoresis and flow cytometry analysis. The phagocytosis of apoptotic neutrophil,its ability to migrate toward a chemoattractant,and its ability of superoxide releasing were also studied in this paper. Results: The rate of apoptosis of human PMN increased as the incubate time prolong. The phagocytosis of neutrophil,its ability to migrate toward a chemoattractant,and its ability of superoxide releasing were all decreased as it was cultured for a longer time. Conclusions: Neutrophil apoptosis could affect its function,the function of apoptotic neutrophil were decreased.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2000年第10期535-538,共4页
Chinese Journal of Immunology
基金
国家自然科学基金!资助项目 (批准号 39770 716 )
关键词
中性粒细胞
趋化
活性氧生成
细胞凋亡
细胞吞噬
Polymorphonuclear neutrophils Apoptosis Phagocytosis Chemotaxis Superoxide releasing
