摘要
目的 探讨外周血单核细胞（PBMC）Toll样受体4（TLR4）在冠心病合并糖尿病中的表达及其意义.方法 分组：健康对照组40例,糖尿病组36例,冠心病组43例,冠心病合并糖尿病组40例.采用SYBR Green Ⅰ实时荧光定量RT-PCR和流式细胞仪检测PBMC TLR-4的表达.结果 与对照组相比,糖尿病、冠心病及冠心病合并糖尿病组的TLR4 mRNA表达水平和TLR4阳性细胞百分比均上调（均P＜0.05）；糖尿病与冠心病两组间差异无统计学意义,而冠心病合并糖尿病组显著高于糖尿病组和冠心病组（均P＜0.05）.Logistic回归分析显示,TLR-4是冠心病并发糖尿病的危险因素（OR =4.048,P＜0.01）.结论 TLR4在糖尿病合并冠心病共同的发病基础中可能起着一定作用.
Objective To study the Relationship between Toll-like receptor 4（TLR4）in Peripheral-blood mononuclear cells（PBMC） and diabetic me]litus（DM） in coronary heart disease（CHD） patients. Methods Control group（n=40）, DM group （rt=36）, CHD group （n=43） and CHD wih DM group （n=40）. The expressions of TLR4 mR- NA and TLR4 protein on PBMC were examined by SYBR Green I real-time quantitative reverse transcription ploymerse chain reaction （RT-PCR） and Flow cytometric analysis respectively. Results compared with control group, the expressions of TLR4 mRNA and TLR4 protein were upgraded in DM group,CHD group and CHD wih DM group,and difference was no statistically significant between DM group and CHD group. Compared with DM group and CHD group, the level of TLR4 increased in CHD wih DM group （P〈0.05）. Logistic regression analysis showed that TLR-4 is the risk factor in the progression of DM in CHD（OR=4.048, P〈O.O1）. Conclusion TLR-4 may play a minor role in the the common oathogenetic basis of CHD wih DM.
出处
《中国心血管病研究》
CAS
2013年第10期775-778,共4页
Chinese Journal of Cardiovascular Research
关键词
冠心病
糖尿病
外周血单核细胞
TOLL样受体4
Coronary heart disease
Diabetes mellitus
Peripheral-blood mononuelear cells
Toll-like Receptor4
