摘要
目的 为有效提高小分子多肽在体诱导CTL应答的能力 ,制备了乙肝治疗性多肽免疫刺激复合物 (ISCOMs) ,并对其形成进行了鉴定。方法 乙肝治疗性多肽ISCOMs采用透析法制备获得 ,并经小分子多肽的SDS PAGE电泳法确定了复合物中小分子多肽的存在 ,采用Bradfords法测定了复合物中多肽的结合量。结果 在电镜下呈典型的蜂巢状亚微型颗粒结构 ,所制备的ISCOMs中多肽的结合量约为 0 .30mg/ml。结论 ISCOMs的成功制备 。
Objective To prepare and characterize immunostimulatory complexes(ISCOMs) from a hepatitis B therapeutic peptide in order to improve the cytotoxic T lymphocyte(CTL) responses induced by short peptide antigen in vivo . Methods The ISCOMs were prepared by dialysis approach, and the formation was observed with electron microscopy. The short polypeptide in the ISCOMs was analyzed with SDS PAGE method and the content of peptide incorporated into the ISCOMs was determined by Bradford's method. Results Typical honeycomb like structure of ISCOMs was observed with electron microscope, and hepatitis B peptide content incorporated into the ISCOMs was approximately 0.30 mg/ml. Conclusion The ISCOMs were successfully prepared from the short polypeptide which founded a basis for the further study on immune features of the complexes.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第10期927-930,共4页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 !(397890 1 0 )
关键词
免疫刺激复合物
制备
鉴定
乙型肝炎
immunostimulating compexes
preparation
characterization
