摘要
目的 观察碱性成纤维细胞生长因子(bFGF)抗局灶性脑缺血/ 再灌注引发神经元凋亡作用。方法 采用线栓法制备大脑中动脉缺血/ 再灌注模型。术后首次即刻分别腹腔注射bFGF和生理盐水，以后每天一次，连用7天。TUNEL 法原位检测缺血后第1、2、3、5、7天脑石蜡切片含DNA 碎片的凋亡细胞。结果 正常组、 假手术组、缺血组对侧半球每张切片有0～8个凋亡细胞，缺血/ 再灌注后缺血区凋亡细胞明显增多，缺血48h达高峰，以后逐渐降低，第7天仍可见凋亡细胞。bFGF组凋亡细胞数显著低于缺血/ 再灌注生理盐水对照组。结论 缺血/ 再灌注后缺血区神经元发生了凋亡，bFGF具有抗凋亡作用。
Objective To ascertain the effect of basic fibroblast growth factor(bFGF) reducing neuronic apoptosis. Methods The animal models of the right middle cerebral artery ischemia/reperfusion was established by suture method in adult wistar rats. The bFGF(100μg/kg) in physiological saline, or physiological saline alone,was injected intraperitoneally immediately after cerebral ischemia, then once daily, for 7 days.We measured brain apoptotic cells exhibiting DNA fragmentation in paraffin sections with the method of a terminal deoxynucleotidylltransferase(TdT)- mediated dUTP- fluorescein nick end- labelling (TUNEL) after ischemia 2 hour/reperfusion for the first,second, third, fifth and seventh day. Results There were 0～8 apoptotic cells per section in normal or sham-operated groups. After cerebral ischemia/reperfusion, the apoptotic neuron number increased significantly in the ischemic region. After 48h, the apoptotic nouron decreased gradually, up to the seventh day, there were also apoptotic neuron. The number of apoptotic neurons in the bFGF- treated group differed signifficantly from that in the non-treated control group (Ｐ<0.05). Conclusion This study suggested that ischemia/reperfusion may induce neuronic apoptosis and bFGF shows the effect of reducing neuronic apoptosis.
出处
《重庆医科大学学报》
CAS
CSCD
2000年第4期345-347,共3页
Journal of Chongqing Medical University
