摘要
利用先前构建的Sollux/Gaoyou DH群体在4种环境下的表型结果和最新遗传图谱,通过条件和非条件QTL定位剖析油菜种子4种主要脂肪酸含量与含油量之间的遗传相关.结果显示,检测到的所有主效、上位性和环境互作QTL效应值均较小,但主效QTL都达到极显著水平(P<0.01).分别检测到7、10、10和5个芥酸、油酸、亚油酸和亚麻酸QTL位点,其中位于A2、A7、A9和C1上的芥酸,C7和A9上的油酸和亚麻酸以及A4、A10、C6、C7和C9上的亚油酸位点为新检测出的微效QTL.条件QTL定位结果表明,OilA9、OilC2和OilC8-1受亚油酸影响较大.OilA4同时受油酸、亚麻酸和芥酸的影响,OilA1、OilA7和OilC8-2所在区间可能存在控制油酸含量的微效基因,对含油量起调控作用.另外,还检测出4个效应值更小的条件QTL新位点,进一步说明含油量性状的复杂性和难以操控性.检出的芥酸和3个18碳脂肪酸的QTL数量远多于已有在芥酸分离群体中定位到的QTL,但效应值均较小,表明在无主效芥酸等位基因分离情况下,使控制芥酸和3种18碳脂肪酸的众多微效基因得以表达.通过标志辅助聚合微效高芥酸等位基因可能对选育工业专用高芥酸油菜品种具有特殊意义,通过标志辅助选择OilA7和OilC8位点的高含油量等位基因可同步提高种子中油酸含量.
Oil content in rapeseed is a complex quantitative trait, which is controlled by multiple genes and influenced by environments. Extensive efforts have been made in mapping of the QTL for this trait since 1995, which indicate that almost all the 19 linkage groups have found the presence of oil QTL in Brassica napus. On the other hand, a large body of QTL information for fatty acid compositions is also available because they play animportant role in the accumulation of oil content. However, the genetic relationships between oil content and individual fatty acid content on QTL level still remains poorly understood. Our main objective was to evaluate the genetic influence of four major fatty acid components on oil content in a population with no allelic segregation on two major erucic acid gene loci in oilseed rape.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2013年第5期504-512,共9页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
浙江省科技计划资助项目(2012C12902-1
2012C32005)
国家自然科学基金资助项目(31171180
31271608)
浙江省基金重点资助项目(Z3100592)
关键词
甘蓝型油菜
含油量
脂肪酸
数量性状基因座
条件QTL定位
Brassica napus
oil content
fatty acid
quantitative trait locus （QTL）
conditional QTL mapping
