摘要
目的探讨重叠综合征患者血清肿瘤坏死因子(tumor necrosis factor,TNF)-α、白细胞介素(interleukin,IL)-6、C反应蛋白(C-reactive protein,CRP)和细胞免疫水平及二者间关系。方法阻塞性睡眠呼吸暂停低通气综合征患者25例(OSAS组)、慢性阻塞性肺疾病患者25例(COPD组)、重叠综合征患者25例(OS组)和对照组25例,4组采用ELISA法检测血清TNF-α、IL-6、CRP水平,采用流式细胞仪检测T淋巴细胞亚群水平。结果 OS组血清TNF-α、IL-6、CRP水平高于对照组、OSAS组和COPD组(P<0.01),CD4+/CD8+低于对照组、COPD组与OSAS组(P<0.01);OS组炎性因子TNF-α、IL-6、CRP与CD4+/CD8+呈明显负相关(r=-0.754,P=0.013;r=-0.503,P=0.027;r=-0.552,P=0.008)。结论重叠综合征患者存在全身性系统性炎症反应,可引起细胞免疫功能紊乱。
Objective To explore the relationship of the levels of inflammation factor tumor necrosis factor a （TNF-α）, interleukin-6 （IL-6） and C-reactive protein （CRP） with cellular immune in the overlap syndrome patients. Methods The levels of serum TNF-α, IL- 6 and CRP were detected by using ELISA technique, and the T-lymphocyte subsets were determined by using flowcytometry method in 25 cases of obstructive sleep apnea syndrome （OSAS group）, 25 cases of chronic obstructive pulmonary disease （COPD group）, 25 cases of overlap syndrome （OS group） and 25 cases of normal control （control group）. Results The levels of IL-6, CRP and TNF-α were higher, and the CD4＋/CD8＋ ratios were lower in OS group than those in the other three groups （P〈0.01）. The levels of TNF-α, IL-6 and CRP were negatively correlated with CD4＋/CD8＋ ratio （r= 0.754, P=0. 013; r=-0. 503, P 0.027; r=-0. 552, P=0. 008）. Conclusion The systemic inflammation reaction exists in the overlap syndrome patients, which causes immunity disorders.
出处
《中华实用诊断与治疗杂志》
2013年第10期973-975,共3页
Journal of Chinese Practical Diagnosis and Therapy
