摘要
探讨金黄色葡萄球菌对氟喹诺酮类的耐药与gyrA基因突变的关系及中药耐药抑制剂对gyrA基因是否具有回复突变作用,以便从分子水平初步探讨复方中药耐药抑制剂的耐药抑制分子机理。用诺氟沙星逐步诱导金黄色葡萄球菌敏感株ATCC29213,建立了MIC值为64μg/mL、128μg/mL和256μg/mL的耐药突变株;PCR扩增标准株、耐药株和中药处理的耐药株gyrA基因,产物回收纯化后送测序公司测序。gyrA基因测序结果用DNAsis软件分析表明,细菌的耐药性程度越高,碱基突变率增加;gyrA基因氨基酸序列突变分析表明,84位亲水性丝氨酸被疏水性缬氨酸取代,与细菌的高水平耐药性密切相关。中药耐药抑制剂对84位和99位氨基酸突变具有回复突变的作用。结论:中药耐药抑制剂对耐药金黄色葡萄球菌gyrA基因具有回复突变作用。
To investigate the Staphylococcus aureus resistance to fluoroquinolones with gyrA gene mu- tation and reverting role of Chinese medicine resistance inhibitor. Methods. susceptible strains of standard Staphrlococcus aureus with drug-induced a series of norfloxacin-resistance were artificially induced to resist fung. PCR amplification for gyrA of resistant strains in the treatment of resistant strains and Chinese med- icine resistance inhibitor, processing gene sequencing and sequence analysis. Result. The results show that., the higher the degree of bacterial resistance, the more mutation rate increases. In gyrA gene amino acid sequence mutation analysis, 84 status hydrophilic serine is replaced by hydrophobic valine, which closely relate to bacterial resistance. Chinese medicine resistance inhibitors have the function of reverse mutations on 84 and 99 amino acid mutated. Conclusion. Herbal drug-resistant inhibitor of high levels of drug-resistant Staphylococcus aureus gyrA gene reverting.
出处
《中国兽医杂志》
CAS
北大核心
2013年第9期3-5,共3页
Chinese Journal of Veterinary Medicine
基金
科技部国际科技合作计划专项基金(2009DFA32350)
