摘要
芦苇适应性广、生物产量高,是一种很有潜力的可再生能源作物,但芦苇质地紧密,难以直接酶解,该研究采用蒸汽爆破对芦苇进行预处理,并对稀硫酸、NaOH及稀H2SO4-蒸汽爆破预处理液及酶糖化液糖组分进行比较分析。结果表明,稀H2SO4-蒸汽爆破预处理效果最好,1%H2SO4浸泡芦苇10h,2.0MPa-120s汽爆处理,预处理糖化率为37.8%,酶解糖化率达82%;糖液组分分析也显示,稀H2SO4-蒸汽爆破预处理能得到更多的可发酵糖,酶解液糖浓度31.22mg/mL,葡萄糖和木糖含量分别占52.75%和43.39%。
Reed is a kind of high biomass plants. It can be used as important raw material for bio-ethanol production. In this research, several pretreatments （steam explode, alkali-catalyzes steam explosion, acid-catalyzes steam explosion） and enzymatic saccharification procedures were studied. Hydrolystates from three pretreated （dilute acid, sodium hydroxide and acid-catalyzes steam explosion） reed were analyzed. The results showed that acid-catalyzes steam explosion was the best pretreatment in terms of resulting reducing sugar concentration in the hydrolysate after enzymatic hydrol- ysis. The conditions of acid-catalyzes steam explosion was pretreated by soak in 1.0% dilute acid, then performed steam explosion in a retention time of 120s and a steam-pressure of 2.0MPa. The highest rate of hydrolysis and enzymatic saccharification were 37.8% and 82%, respectively. The analysis of hydrolysate for pretreatment and enzymatic saccharification from reed indicated that acid-catalyzes steam explosion pretreatment could produced more fermentable sugar, and the contents of the glucose and xylose in enzymatic hydrolysate were 52.75% and 43.39%, respectively.
出处
《中国酿造》
CAS
2013年第9期71-74,共4页
China Brewing
基金
现代农业产业技术体系资助项目(CARs.19.E26)
长沙市培育战略性新兴产业科技支撑资金专项(K1205005-11-1)
关键词
芦苇
纤维素
蒸汽爆破预处理
酶糖化
reed
cellulose
steam explosion pretreatment
enzymatic saccharification
