摘要
目的研究老鼠簕生物碱A及其2个乙酰化衍生物对免疫性肝纤维化大鼠的保护作用。方法采用腹腔注射0.5ml的猪血清制备大鼠免疫性肝纤维化的模型,分别给予老鼠簕生物碱A、单乙酰化物(AcO-BOA)、双乙酰化物(TC-3),检测血清中丙氨酸氨基转移酶、天冬氨酸氨基转移酶的活性,以及用酶联免疫吸附测定法检测血清透明质酸和层粘连蛋白的含量,同时观察肝组织的病理变化。结果与空白对照组相比,模型组的丙氨酸氨基转移酶、天冬氨酸氨基转移酶、透明质酸和层粘连蛋白均有明显升高(P<0.05);与模型组相比,3个化合物均能不同程度地降低丙氨酸氨基转移酶、天冬氨酸氨基转移酶的活性和透明质酸和层粘连蛋白的含量(P<0.05);病理结果显示,给药组均能减轻猪血清造成的肝损伤。结论 3个化合物具有不同程度抗肝纤维化形成的作用,且有保肝的作用,其机制可能与抑制细胞外基质的胶原合成有关。
OBJECTIVE To research the protective effects of Acanthus ilicifolius alkaloids A and its two derivatives in the rats with immune hepatic fibrosis. METHODS Immune hepatic fibrosis model was established by intraperitoneal administration porcine serum. SD rats were treated intragastric administration with [4-hydroxy-2（3H）-benzoxazolone,HBOAl , [4-acetoxy-2（3H）-benzox- azolone,AcO-BOA ] , （3-acetyl-4-acetoxy-2-benzoxazolone,TC-3）. The activities of ALT, AST in serum and the levels of HA and LN in serum were measured. RESULTS Compared with the normal control group, the activities of ALT, AST and the levels of HA and LN in the model group were significantly increased. Compared with the model group, animals treated with the HBOA, AcO-BOA or TC-3 could decrease the activities of ALT and AST and the levels of HA and LN markedly. Histopathological results showed that treat- ment groups could relieve the porcine serum-induced liver damage. CONCLUSION HBOA and its derivatives have protective effect on the rats with immune-mediated hepatic fibrosis.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2013年第20期1744-1747,共4页
Chinese Pharmaceutical Journal
基金
广西自然科学基金资助项目(编号2009AM4019)
关键词
免疫性肝纤维化
老鼠簕生物碱A
猪血清
immune hepatic fibrosis
Acanthus ilicifolius alkaloids A
porcine serum
