摘要
为建立环磷酰胺诱导隐性弓形虫感染小鼠复发为急性弓形虫病的模型,20只ICR小鼠腹腔注射弓形虫Prugniaud株包囊10个/只鼠,10只小鼠注射等量无菌PBS。2个月后给所有小鼠每天腹腔注射环磷酰胺100mg/kg,连续注射14d。隔天监测小鼠体重、全血中白细胞数和弓形虫基因,同时取濒死小鼠肝、脾和肺组织,进行HE染色和免疫荧光检测。结果发现,感染小鼠注射环磷酰胺后第8天开始出现急性弓形虫病的症状;与试验前比较,小鼠体重和白细胞数明显下降;第10天时全血中再次发现弓形虫基因;第10天后濒死小鼠组织中检测到弓形虫基因,HE染色发现明显病理损伤,免疫荧光检测到弓形虫抗原。证明环磷酰胺可成功诱导隐性弓形虫感染小鼠复发为全身性的急性弓形虫病。
To study the model that Toxoplasrna gondii inapprent infection mice is reactivated to acute toxoplasmosis by cyclophosphamide （CTX）, 20 ICR mice were injected with cysts intraperitoneally, and a mouse was got 10 cysts of T. gondii Prugniaud strain （PRU）. 10 ICR mice were injected with partes aequales sterile PBS. After two months, all mice were injected intraperitonelly with CTX, 100 mg/kg, d for 14 days. The body weight, number of WBC and T. gondii gene in the blood on alternate days were moni- tored,at the same time,the liver, spleen and lung from the mice on day 10 post injection were made for HE staining and immunofluorescence staining. As a result, the infected mice appeared the symptoms of acute toxoplasmosis after 8 days post injection. Compared with the mice befor experimental, the body weight and the number of blood WBC were descendent obviously, and T. gondii genes were seen on day 10 post injec- tion. T. gondii gene could be tested in liver, spleen and lung of the dead mice after 10 days post injection. The histopathological damages were observed by HE staining, and T. gondii antigen could be detected by immunofluorescence in the mice on day 10 post injection. Cyclophosphamide could make successfully inapparent infection mice reactivate acute toxoplasmosis.
出处
《动物医学进展》
CSCD
北大核心
2013年第10期46-49,共4页
Progress In Veterinary Medicine
基金
贵州省科学技术基金(黔科合J字[2011]2278号)
