摘要
为了进一步研究辣椒子叶再生体系的影响因素,以辣椒王种子为试材,诱导无菌苗,取子叶作为外植体,探讨了不同浓度6-BA对辣椒子叶诱导不定芽的影响,并进行了不同浓度GA3与6-BA组合对不定芽伸长效果的研究。结果表明:辣椒子叶在MB5+0.8mg·L-1 IAA+8.0mg·L-1 6-BA+4.0mg·L-1 AgNO3培养基上诱导不定芽效果最好;MB5+0.8mg·L-1 IAA+5.0mg·L-1 6-BA+2.0mg·L-1 GA3+4.0mg·L-1 AgNO3培养基有利于不定芽的伸长。
In order to further study the influence factors of cotyledon regeneration system of in vitro Capsicum annuum L. , ＇Lajiaowang＇ seeds were used to induce in vitro plantlets（Capsicum annuum L. ）. The differences of adventitious buds induction were compared among the different concentrations of 6-BA. In the meanwhile, the effects of the different concentrations of GAa and 6-BA combinations on elongation were analyzed. The re- sults showed that the best medium for adventitious buds induction by hypocotyls was MB5＋0.8mg·L^-1IAA＋8.0mg·L^-16-BA＋4.0mg·L^-1AgNO3. The suitable medium for the adventitious buds elongation was MB5＋0.8mg·L^-1IAA＋5.0mg·L^-16-BA＋2.0mg·L^-1GA3＋4.0mg·L^-1AgNO3.
出处
《黑龙江农业科学》
2013年第10期12-15,共4页
Heilongjiang Agricultural Sciences
基金
福建农林大学本科教学改革资助项目(No.111412099)
关键词
辣椒
子叶
不定芽诱导
伸长
Capsicum annuum L.
cotyledon
adventitious buds induction
elongation
