摘要
以油菜品种 ０８５（抗菌核病）与湘油 １３号杂交得到的 Ｆ２代作为作图群体，以 ＲＡＰＤ标记 来构建油菜连锁图谱、通过对３００个１０－ｍｅｒ随机引物的筛选，共获得２００个多态性ＰＡＰＤ位 点。经Ｍａｐｍａｋｅｒ／ＥＸＰ３．０软件处理，构建了１张含１９３个标记位点、１９个连锁群、覆盖长度为 １３２４ｃＭ的连锁图谱、在此基础上利用 Ｍａｐｍａｋｅｒ／ＱＴＬ１．１将抗菌核病基因定位在第四、八和 十四３个连锁群上，即：Ｓｃ１、Ｓｃ２和Ｓｃ３。其中Ｓｃ２解释表型变异的２９％，相对Ｓｃ１和Ｓｃ３而言，Ｓｃ２具有主效基因的特征。 ３个 ＱＴＬｓ的联合贡献率约为 ５６％。
Scterotinia scterotiorum (Lib.) de by is a world-wide disease, which seriously decreases the yield and the quality of oil of rapeseed. Mapping resistance genes of S. scterotiorum is of vital effect to breed new elite cultivars resisting the disease. In this paper, we reported that a RAPD linkage map was constructed, including 193 RAPD markers, 19 linkage groups and covering 1 324 cM, by using Mapmaker 3.0. On the basis of map, and resistance genes, Sc1, Sc2 and Sc3, were located in 4th, 8th and 18th linkage groups. Among three QTLs, Sc2 has the characteristics of major gene with explained 29%. The three QTLs together explained about 56% of phenotypic variation.
关键词
甘蓝型油菜
连锁图构建
抗菌核病性状
QTL定位
Brassica napus
linkage groups
S. scterotiorum resistance character, QTL mapping
