摘要
利用 Nd:YAG激光 (波长 1 .0 6μm,功率 3 0 W,4 0 W)照射雨生红球藻的营养细胞 ,辐照时间为1 0 sec,3 0 sec,60 sec。辐照后进行生理生化特性的测定分析。实验结果表明 ,经激光辐照后 ,藻细胞在运动能力 ,生长速率 ,色素含量及厚壁孢子的形成等方面均有不同程度的变化。其中 ,低剂量辐照组 (功率 3 0 W,辐照1 0 sec,3 0 Sec和 4 0 W,辐照 1 0 sec)的生长期延长 ,生长速率较出发株最高增加 1 1 .4 % ,培养 1 2天时的细胞密度最高增加 2 9.7%。高剂量辐照 (功率 3 0 W,辐照 60 sec,4 0 W,辐照 3 0 sec)对生长有抑制作用 ,但可加速虾青素的累积和孢子的形成 ,培养 1 2天时虾青素含量与孢子发生率的增幅最高分别达 3 5.5%和 3 2 .1 %。
Nd:YAG Laser (wavelength 1.06μm, power 30w, 40w)was used to irradiate the vegetative cells of Haematococcus pluvialis, and with irradiate does of 10 sec, 30sec,60sec.The physiological and biochemical characteristic of cells irradiated were determined and analysed. The experimental results show that the motility of cells, growth rates, pigment content and formation of akinets were varied at different degrees after irradiation. Among these, the growth were stimulated and growth phase were prolonged at low irradiate dose(30w, 10sec,30sec;40w, 10sec,), of which, the growth and the cell density were increased most by 11.4% and 29.7% respectively. The does of high irradiate(30w,60sec;40w,30sec) inhibitated growth, but stimulated astaxanthin accumulation and akinete formation. of which, the astaxanthin content and the percentage of akinte were increased most by 35.5% and 32.1% respectively.
出处
《激光生物学报》
CAS
CSCD
2000年第3期176-180,共5页
Acta Laser Biology Sinica
基金
福建省自然基金项目!( C970 2 0 )
福建省教委资助项目
