摘要
考察了５０Ｌ发酵罐中青春双歧杆菌在混合胶类物质的果蔬汁底料中的生长及其溶氧率、ｐＨ值、可溶性固形物等变化规律，就培养后的双歧杆菌制备活菌固体制剂的工艺方法进行比较。结果表明胶类物质能缓冲培养基质中酸性环境，减少发酵和制剂过程中空气氧对菌细胞的毒害，干燥后的固体制剂中活菌回收率高，贮存的稳定性增加。提示了明胶和黄原胶在配料干燥后对菌细胞的包埋及保护作用。并制定出关于青春双歧杆菌保健片或粉料的简便生产工艺。
The effect of gelatin on anaero-cultivation of Bifidobacterium adolescent is and stabilization of its living cell in dried preparation was investigared. It was shown that the well-distributed mixture of gelatin and medium could have a cushioning effect on pH reduction resulting from acid-production while incubating, and the dried mixture comprising living cell of bifidobacterium adolescentis was prepared, in which the stability and viability of cell come from cultures of gelatin mixture was increased compared with that without gelatin mixture. It was shown that oxygen toxicity to anaerobic cell was well reduced because of cell immobilization by the gel mixure after drying with saccharide substance , and then fermentable dynamics in 50L fermentor was tested as well as the relationship of growth kinetics, pH, dissolved oxygen rate, and content of soluble solids.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第6期433-436,共4页
Microbiology China
基金
深圳市科技局基金!(No.1998-48-26)
关键词
微胶囊
双歧杆菌
固定化
发酵
厌氧发酵
Microcapsule, Bifidobacterium, Fermentation, Immobilization$
