摘要
利用Cd2+螯合树脂,对噬菌体随机十二肽库进行Cd2+结合肽筛选,并将亲和力较强的结合肽展示在酵母EBY100表面,以期得到耐受和吸附能力具佳的工程菌,为重金属的生物修复提供新的思路。通过筛选共得到6条不同的Cd2+结合肽,其中有2条对Cd2+亲和力较强,将其串联展示于酵母细胞表面。与对照菌相比,酵母工程菌对Cd2+、Ni 2+的吸附率显著提高。酵母工程菌对Cd2+的吸附率随诱导时间的增加而增加,诱导24h时最高,达30.4%,此时酵母工程菌对Ni 2+的吸附率达到32.9%。
Cd^2＋ binding peptides were effectire enriched from the commercially phage 12 random peptide library,and six different Cd2＋ binding peptides were obtained,two which had strong affi- nity were selected and connected together in series and displayed on yeast surface by gene recom- bination. The recombinant yeasts showed significant higher adsorption rate for Cd^2＋ and Ni^2＋ as compared with the control. The recombinant yeasts adsorption rate for Cd2＋ increased with the in- crease of the induction time, when the induction time was 24 h, the adsorption rate for Cd2＋ reached the maximum （30.4%） ,and the adsorption rate for Nia＋ reached 32.9%.
出处
《河南农业科学》
CSCD
北大核心
2013年第9期142-145,共4页
Journal of Henan Agricultural Sciences
基金
国家"863"计划项目(2006AA06Z353)
关键词
重金属Cd2＋结合肽
噬菌体随机肽库
展示技术
酵母
heavy metal
Cd2＋ binding petides
phage random peptide library
display technology
yeast
