摘要
目的监测哮喘患者血清IL-25、ECP及IL-27的表达水平,探讨其水平变化及其在哮喘发病中的作用。方法收集成人哮喘患者急性发作期、缓解期及健康成人的静脉血标本,用ELISA法测定血清IL-25、ECP、IL-27的水平。检测哮喘患者急性发作期的肺功能FEV1、PEF。结果支气管哮喘急性发作期组血清IL-25及IL-27水平显著高于缓解期组及健康对照组,差异有统计学意义(P<0.05);2组缓解期组与健康对照组比较差异无统计学意义;哮喘急性发作期组血清ECP水平显著高于缓解期组及健康对照组,差异有统计学意义(P<0.05)。哮喘急性发作期患者血清IL-25及IL-27水平均与ECP之间呈正相关。哮喘急性发作期患者血清IL-25与IL-27水平与肺功能FEV1/FEV1预计值、PEF/PEF预计值呈负相关。结论哮喘成人血清IL-25及IL-27水平能反应哮喘气道炎症活动情况及疾病严重程度。
This study designed to monitor the expression of IL-25,IL-27 and eosinophils cationic protein（ECP） in serum of asthma patients,for evaluating their changes and roles in the pathogenesis of asthma.Serum samples were prepared from asthma patients in episode period,asthma patients in remission period,and healthy controls.Then double antibody sandwich ELISA method was used to determine and compare serum levels of IL-25,IL-27 and ECP.And pulmonary function was measured in the episode group.Results showed that serum IL-25 and IL-27 of episode group were markedly higher than those of remission group and control group（P 0.05）,but there was no significant difference between remission group and control group（P 0.05）.Serum ECP of episode group was markedly higher than that in remission group and control group（P 0.05）,but there was no significant difference between remission group and control group（P 0.05）.Furthermore,in episode group,serum IL-25 and IL-27 were positively correlated with ECP,but negatively correlated with FEV1 or PEF.In conclusion,serum levels of IL-25 and IL-27 in asthma adults can reflect the inflammation activities and severity of airway in asthma.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2013年第10期912-914,共3页
Immunological Journal
