摘要
目的:利用复合多因素刺激方法复制肝郁脾虚证动物模型,探讨肝郁脾虚证动物模型复制方法和病理生理改变。方法:利用复合多因素造模方法,并观察造模前后、给药前后大鼠行为学及血浆促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、可的松(CORT)、精氨酸加压素(AVP)含量的变化。结果:模型对照组与正常对照组、逍遥散组、酮康唑组地塞米松组比较,CRH显著升高,差异有统计学意义或显著性差异(P<0.05,P<0.01);模型对照组与正常对照组、逍遥散组、酮康唑组、地塞米松组比较,CORT显著升高,有显著性差异(P<0.01)。同时,模型对照组与正常对照组、逍遥散组、酮康唑组和地塞米松组比较,ACTH含量升高,差异有统计学意义或有非常显著性差异(P<0.05,P<0.01);模型对照组与正常对照组、逍遥散组、酮康唑组和地塞米松组比较,AVP含量降低,有显著性差异(P<0.01)。结论:CUMS造模方法可以成功复制肝郁脾虚证模型,肝郁脾虚证的病理生理与HPA轴功能紊乱有关,拮抗HPA轴亢进是逍遥散治疗肝郁脾虚证的机制之一。
Objective： The present study was designed to duplicate animal model of depression with LDSD （Liver-depression and spleen-deficiency） utilizing approach d multl-factor stimulus, and we have researched the approach of animal model of LDSD and its pathophysiological mechanism. Methods： Before and after duplicated, it was determined that behaviors and the levels of plasma CRH, ACTH, CORT, AVP. Results： Compared to groups of the control, Xiao-yao-san, Ketoconazole, and I）examethasone, the CRH of model pup was increased significantly （P 〈0. 05, P 〈0. 01, respectively） ; compared to groups of the control, Xiao-yao- san, Ketoconazola, and D examethasone, the CORT of model group was increased significantly （P 〈0.01, respectivdy） ; mean- while compared to groups of the control, Xiaoyaosan, Ketoconazole, and Dexamethasone, the ACTH of model group was increased significantly （ P 〈0. 05, P 〈0.01, respectively） ; but compared to groups of the control, Xiao-yao-san, Ketoconazole, and Dexam- ethasone, the AVP of model group was decreased significantly （P 〈 0. 01, respectively） . Conclusion： The experiment suggests that it is possible and successful for animal modal of LDSD in CUMS and its pathophysiological changes is related to the irregulars of HPA. Adjustment of function of HPA can be pharmacodynamic mechanism of Xiao-yao-san, in which cured LDSD through.
出处
《成都中医药大学学报》
2013年第3期10-14,23,共6页
Journal of Chengdu University of Traditional Chinese Medicine
基金
贵州省科学技术基金项目(编号:黔科合J字(2011)2302)
