摘要
从DPPH自由基、羟自由基（·OH）清除能力以及对Cu2＋-H2O2/AAPH体系诱导蛋白氧化损伤及羰基化的抑制4个方面对草质素的体外抗氧化活性进行观察。结果表明：10～100μmol/L的草质素能够显著清除DPPH自由基和·OH，半抑制浓度（IC50值）分别为49.28、219.20μmol/L。同时，草质素呈浓度依赖方式抑制Cu2＋-H2O2及AAPH诱导的蛋白氧化损伤及羰基化。草质素具有较强清除自由基和抑制蛋白质氧化的能力。
The in vitro antioxidant activity of herbacetin was investigated in terms of scavenging activities against DPPH and hydroxyl free radicals and inhibitory effects against oxidative protein damage and carbonylation induced by Cu2＋- H2O2 or AAPH. At a concentration between 10 μmol/L and 100 μmol/L, herbacetin exerted a marked scavenging effect on DPPH and hydroxyl free radicals, with respective IC50 values of 49.28 μmol/L and 219.2 μmol/L. Meanwhile, herbacetin could significantly inhibit oxidative protein damage and carbonylation induced by Cu2＋-H2O2 or AAPH in a dose-dependent manner. This study concludes that herbacetin has a strong ability to scavenge free radical and inhibit oxidative protein damage.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第17期106-110,共5页
Food Science
关键词
草质素
自由基
蛋白损伤
羰基化
抗氧化
herbacetin
free radical
protein damage
carbonylation
antioxidantactivity
