摘要
根据GenBank发布的坦布苏病毒Bagaza毒株E基因的保守序列设计1套引物,建立了环介导等温扩增快速检测鸭坦布苏病毒的方法。该检测方法的敏感性可达10拷贝/μL;对鸭坦布苏病毒山东分离株的检测呈阳性,而对鸭瘟病毒、禽流感病毒(H9亚型)、鸭新城疫病毒、传染性法氏囊病病毒、减蛋综合征病毒扩增结果均为阴性;应用该方法对山东省各地采集的82份疑似病料进行检测,可检出72份阳性。表明本试验所建立的环介导等温扩增检测方法灵敏性高、特异性强、操作简便快速,可用于鸭坦布苏病毒病的临床诊断和流行病学调查。
A loop-mediated isothermal amplification（LAMP）assay was developed for the rapid detection of Tembusu virus（TMUV）using a set of specific primers targeting on the E gene conserved region of TMUV Bagaza strain published in GenBank.The assay has the lowest detection limit being 10 copies/μL,and one strain of TMUV of Shandong isolates were positive,and there was no cross-reactivity with duck plagues virus（DPV）,avian influenza virus（AIV）subtype H9,Newcastle disease virus（NDV）,infectious bursal disease virus（IBDV）and egg drop syndrome virus（EDS-76）.Application of the method,82 suspicious samples collecting from Shandong province was tested and 72 samples was positive.These results suggested that the LAMP assay with high sensitivity,strong specificity,simple operation could be used as a method for the diagnosis and detection of clinical cases,and molecular epidemiological investigation of duck TMUV.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第9期1329-1332,共4页
Chinese Journal of Veterinary Science
基金
现代农业产业技术体系建设专项基金资助项目(CARS-43-34)
