摘要
目的探讨肿瘤干细胞标记物Lgr5和CD133在卵巢癌中的表达和意义。方法利用免疫组织化学方法,观察33例卵巢浆液性乳头状腺癌、6例正常卵巢组织和6例卵巢浆液性囊腺瘤中Lgr5和CD133的表达情况。结果Lgr5在卵巢癌组织中阳性表达率为78.7%,与正常卵巢组织和浆液性囊腺瘤相比有显著性差异(P<0.05)。且随着肿瘤分化程度不同,从高分化到低分化Lgr5阳性表达率逐渐提高。CD133在卵巢癌中阳性表达率为57.5%,与正常卵巢组织相比有显著差异(P<0.05),但与浆液性囊腺瘤无差异(P>0.05)。随着肿瘤分化程度的不同,从高分化到低分化CD133阳性表达率逐渐增高。Lgr5与CD133在卵巢癌组织中的表达呈正相关,即随着Lgr5表达的上调,CD133的表达也呈现增强趋势。结论 Lgr5和CD133有可能成为卵巢浆液性乳头状腺癌干细胞的表面标记物。
Objective To investigate The expression and significance of LGR5 and CD133 as the stem cell markers on the ovarian cancer. Methods We used 33 cases of ovarian papillary serous adenocarcinoma as research objects and 6 cases of normal ovarian tissue and 6 cases of ovarian serous cystadenoma as control to detect the expression of Lgr5 and CD133,stem cell markers,by immunohistochemistry staining. Results In cases of ovarian cancer, Lgr5 was expressed in tumor cells, positive rate was 78.7 %. The expression of Lgr5 in ovarian cancer had significant difference compared with normal ovarian tissue or with ovarian serous cystadenoma （P〈0.05）. The results suggested that the expression level of Lgr5 was gradually upregaulated with the increasing of tumor malignant degree. In cases of ovarian cancer, positive rate of CD133 was 57.5% and there was significant difference compared with normal ovarian tissue（P〈0.05）, however no difference with ovarian serous cystadenoma（P）0.05）. The expression level of CD133 was gradually in creased with reduce of tumor differentiation degree. There was positive correlation between the expression of Lgr5 and CD133 in ovarian cancer （r=0.71,P〈0.01） ,which meant the expression of CD133 was increased with the up regulation of Lgr5. Conclusion It is possible for Lgr5 and CD133 to work as surface markers of ovarian serous papillary adenocarcinoma stem cells.
出处
《中国实验诊断学》
2013年第9期1598-1601,共4页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技厅自然科学基金资助(201015198)
吉林省卫生系统创新人才第三层次和吉林省卫生厅项目资助(2012Z071)
